Nematicidal activity of crude extract from Torreya grandis against root-knot nematode Meloidogyne enterolobii and preliminary insights into its mechanism

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-09-30 DOI:10.1016/j.indcrop.2025.121986

Peng Feng, Cai Xia Zhang, Xin Sen Zhang, Hui Xia Li, Kun Liu, Chen Zhou

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Abstract

Root-knot nematodes like Meloidogyne enterolobii cause severe crop losses, yet chemical nematicides pose environmental and health risks. This study explored the nematicidal activity and mechanism of Torreya grandis crude extract. In vitro assays showed 10.00 mg/ml and 5.00 mg/ml extracts killed 100 % of J2 juveniles within 12 and 24 h, respectively, with 10.00 mg/ml strongly inhibiting egg hatching (only 4 juveniles in 10 days) and triggering the highest avoidance response (index 0.47). The LC₅₀ values decreased over time, while LT₅₀ prolonged with lower concentrations. Greenhouse trials revealed the extract reduced tomato root-knot index, with a maximum control efficacy of 30.23 %. Mechanistically, it inhibited nematode acetylcholinesterase, carboxylic acid esterase, superoxide dismutase, and catalase activities. These results highlight T. grandis crude extract as a promising eco-friendly nematicide, laying the groundwork for developing plant-derived alternatives to chemical nematicides.

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香榧粗提物对肠根结线虫的杀线虫活性及其机制初步探讨

肠曲根结线虫等根结线虫造成严重的作物损失，而化学杀线虫剂则构成环境和健康风险。本研究探讨了香榧粗提物的杀线虫活性及其机理。体外实验结果表明，10.00 mg/ml和5.00 mg/ml提取物分别在12 h和24 h内杀死J2幼鱼100% %，其中10.00 mg/ml提取物强烈抑制卵孵化（10 d内仅4只），并引发最高的回避反应（指数为0.47）。LC₅0的值随着时间的推移而降低，而LT₅0的值随着浓度的降低而延长。温室试验表明，该提取物可降低番茄根结指数，最大防治效果为30.23% %。机制上，它能抑制线虫乙酰胆碱酯酶、羧酸酯酶、超氧化物歧化酶和过氧化氢酶的活性。这些结果表明，大叶菊粗提物是一种很有前景的生态友好型杀线虫剂，为开发植物源性的化学杀线虫剂替代品奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.