Chain-based precision amplification biosensor for microRNA detection based on dCas13a system and iridium complex

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-09-29 DOI:10.1016/j.snb.2025.138881

Yiming Zhang , Zhi Chen , Songrui Wei , Yujun Zhang , Yingxia Liu , Hui Li , Jing Wang , Qi Gao , Defa Li , Han Zhang , Zhongjian Xie

{"title":"Chain-based precision amplification biosensor for microRNA detection based on dCas13a system and iridium complex","authors":"Yiming Zhang , Zhi Chen , Songrui Wei , Yujun Zhang , Yingxia Liu , Hui Li , Jing Wang , Qi Gao , Defa Li , Han Zhang , Zhongjian Xie","doi":"10.1016/j.snb.2025.138881","DOIUrl":null,"url":null,"abstract":"<div><div>Considering the close association between the abnormal expression of multiple microRNAs (miRNAs) and various diseases, multi-channel detection of miRNAs holds significant importance for disease diagnosis. However, traditional miRNA detection methods fall short of simultaneously achieving the required specificity, sensitivity, and multi-channel capability. To address this challenge, we developed a chain-based precision amplification photoelectrochemical biosensor for the simultaneous detection of multiple miRNAs, based on dCas13a system and iridium complex. The precise recognition of miRNA is achieved by the dCas13a-crRNA complex and then converted into a significant photocurrent response from iridium nanocomposite attached to a long double-stranded nucleic acid chain, thereby enabling signal amplification, namely chain-based precision amplification. The dCas13a-crRNA complex is modified onto a multi-channel electrode to simultaneously recognize and bind multiple target miRNAs. The iridium complex is modified on the surface of energy-level-matched quantum dots to suppress carrier recombination, and Au nanoparticles are further modified on the surface of the quantum dots, thereby constructing a nanocomposite for efficient photoelectric conversion. A double-stranded sequence is constructed at the 3′ end of the miRNA to increase the attachment sites for the nanocomposite. The biosensor demonstrates exceptional specificity and sensitivity, capable of distinguishing single-base differences with a detection limit as low as the aM level, benefiting from the chain-based precision amplification. Furthermore, clinical sample testing underscores the practical applicability of the biosensor in clinical diagnostics. This multi-channel chain-based precision amplification biosensor offers a highly valuable and innovative approach for the simultaneous detection of multiple miRNAs, with considerable potential for clinical diagnostic applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138881"},"PeriodicalIF":3.7000,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925400525016570","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Considering the close association between the abnormal expression of multiple microRNAs (miRNAs) and various diseases, multi-channel detection of miRNAs holds significant importance for disease diagnosis. However, traditional miRNA detection methods fall short of simultaneously achieving the required specificity, sensitivity, and multi-channel capability. To address this challenge, we developed a chain-based precision amplification photoelectrochemical biosensor for the simultaneous detection of multiple miRNAs, based on dCas13a system and iridium complex. The precise recognition of miRNA is achieved by the dCas13a-crRNA complex and then converted into a significant photocurrent response from iridium nanocomposite attached to a long double-stranded nucleic acid chain, thereby enabling signal amplification, namely chain-based precision amplification. The dCas13a-crRNA complex is modified onto a multi-channel electrode to simultaneously recognize and bind multiple target miRNAs. The iridium complex is modified on the surface of energy-level-matched quantum dots to suppress carrier recombination, and Au nanoparticles are further modified on the surface of the quantum dots, thereby constructing a nanocomposite for efficient photoelectric conversion. A double-stranded sequence is constructed at the 3′ end of the miRNA to increase the attachment sites for the nanocomposite. The biosensor demonstrates exceptional specificity and sensitivity, capable of distinguishing single-base differences with a detection limit as low as the aM level, benefiting from the chain-based precision amplification. Furthermore, clinical sample testing underscores the practical applicability of the biosensor in clinical diagnostics. This multi-channel chain-based precision amplification biosensor offers a highly valuable and innovative approach for the simultaneous detection of multiple miRNAs, with considerable potential for clinical diagnostic applications.

查看原文本刊更多论文

基于dCas13a系统和铱配合物的microRNA检测链式精密扩增生物传感器

由于多种microRNAs （miRNAs）的异常表达与多种疾病密切相关，因此多通道检测miRNAs对疾病诊断具有重要意义。然而，传统的miRNA检测方法无法同时达到所需的特异性、灵敏度和多通道能力。为了解决这一挑战，我们开发了一种基于dCas13a系统和铱配合物的链式精密放大光电化学生物传感器，用于同时检测多种mirna。通过dCas13a-crRNA复合体实现对miRNA的精确识别，并将铱纳米复合材料附着在长双链核酸链上转化为显著的光电流响应，从而实现信号扩增，即基于链的精确扩增。dCas13a-crRNA复合物被修饰到一个多通道电极上，以同时识别和结合多个目标mirna。在能级匹配的量子点表面修饰铱配合物抑制载流子复合，在量子点表面进一步修饰金纳米粒子，从而构建高效光电转换的纳米复合材料。在miRNA的3'端构建双链序列以增加纳米复合材料的附着位点。该生物传感器具有卓越的特异性和敏感性，能够区分单碱基差异，检测限低至aM水平，受益于基于链的精确扩增。此外，临床样品测试强调了生物传感器在临床诊断中的实际适用性。这种基于多通道链的精密扩增生物传感器为同时检测多种mirna提供了一种非常有价值的创新方法，具有相当大的临床诊断应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.