A microfluidic device integrating magnetic stirring with tangential flow for high-efficiency isolation of small extracellular vesicles

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-09-30 DOI:10.1016/j.snb.2025.138898

Hao Zhong , Zeyin Mao , Xuwen Li , Anni Deng , Yihao Zhu , Shihong Wang , Leyang Huang , Jianxin Zhao , Wenqi Lv , Yixuan Shi , Qin Huang , Yabo Zhai , Chenzhou Hui , Xianbo Luo , Li Ma , Rongxin Fu , Xiongjun Ye , Guoliang Huang

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Abstract

Small extracellular vesicles (sEVs), which are ubiquitously present in bodily fluids, hold significant promise as biomarkers for disease diagnosis and targeted therapy. However, their isolation remains challenging due to their small size (typically < 200 nm) and the complexity of biological fluids. This study presents a novel microfluidic strategy integrating Magnetic Stirring with Tangential Flow Filtration (MS-TFF). The automated, portable device (palm-sized, 12.5 cm × 11.1 cm × 6.25 cm) features user-friendly operation: inserting the chip into a slot and closing the lid initiates automated sEV extraction. The system processes 10 mL of morning urine or cell culture supernatant within ∼40 min. Compared to ultracentrifugation (UC), MS-TFF reduces processing time from ∼4 h to ∼40 min while achieving a 6-fold higher sEV yield. Clinical validation using urine from 16 subjects (6 healthy/10 prostate cancer patients) enabled quantitative *KLK3/PCA3* RNA biomarker detection, achieving AUC = 0.93 for cancer diagnosis, confirming its diagnostic potential.

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一种结合磁搅拌和切向流的高效分离细胞外小泡的微流控装置

小细胞外囊泡（sev）普遍存在于体液中，作为疾病诊断和靶向治疗的生物标志物具有重要的前景。然而，由于它们的体积小（通常为200纳米）和生物流体的复杂性，它们的分离仍然具有挑战性。提出了一种结合磁搅拌和切向流过滤（MS-TFF）的微流体策略。这个自动化的便携式设备（手掌大小，12.5厘米× 11.1厘米× 6.25厘米）具有用户友好的操作特点：将芯片插入插槽并关闭盖子即可自动提取sEV。该系统在40分钟内处理10ml晨尿或细胞培养上清。与超离心（UC）相比，MS-TFF将处理时间从~4小时减少到~40分钟，而sEV收率提高了6倍。使用16名受试者（6名健康/10名前列腺癌患者）的尿液进行临床验证，实现了定量的 KLK3/PCA3 RNA生物标志物检测，实现了癌症诊断的AUC = 0.93，证实了其诊断潜力。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.