{"title":"A Microfluidic Device Integrating Magnetic Stirring with Tangential Flow for High-Efficiency Isolation of Small Extracellular Vesicles","authors":"Hao Zhong, Zeyin Mao, Xuwen Li, Anni Deng, Yihao Zhu, Shihong Wang, Leyang Huang, Jianxin Zhao, Wenqi Lv, Yixuan Shi, Qin Huang, Yabo Zhai, Chenzhou Hui, Xianbo Luo, Li Ma, Rongxin Fu, Xiongjun Ye, Guoliang Huang","doi":"10.1016/j.snb.2025.138898","DOIUrl":null,"url":null,"abstract":"Small extracellular vesicles (sEVs), which are ubiquitously present in bodily fluids, hold significant promise as biomarkers for disease diagnosis and targeted therapy. However, their isolation remains challenging due to their small size (typically < 200<!-- --> <!-- -->nm) and the complexity of biological fluids. This study presents a novel microfluidic strategy integrating Magnetic Stirring with Tangential Flow Filtration (MS-TFF). The automated, portable device (palm-sized, 12.5<!-- --> <!-- -->cm × 11.1<!-- --> <!-- -->cm × 6.25<!-- --> <!-- -->cm) features user-friendly operation: inserting the chip into a slot and closing the lid initiates automated sEV extraction. The system processes 10<!-- --> <!-- -->mL of morning urine or cell culture supernatant within ~40<!-- --> <!-- -->minutes. Compared to ultracentrifugation (UC), MS-TFF reduces processing time from ~4<!-- --> <!-- -->hours to ~40<!-- --> <!-- -->minutes while achieving a 6-fold higher sEV yield. Clinical validation using urine from 16 subjects (6 healthy/10 prostate cancer patients) enabled quantitative <sup>⁎</sup>KLK3/PCA3<sup>⁎</sup> RNA biomarker detection, achieving AUC = 0.93 for cancer diagnosis, confirming its diagnostic potential.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"37 1","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.snb.2025.138898","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Small extracellular vesicles (sEVs), which are ubiquitously present in bodily fluids, hold significant promise as biomarkers for disease diagnosis and targeted therapy. However, their isolation remains challenging due to their small size (typically < 200 nm) and the complexity of biological fluids. This study presents a novel microfluidic strategy integrating Magnetic Stirring with Tangential Flow Filtration (MS-TFF). The automated, portable device (palm-sized, 12.5 cm × 11.1 cm × 6.25 cm) features user-friendly operation: inserting the chip into a slot and closing the lid initiates automated sEV extraction. The system processes 10 mL of morning urine or cell culture supernatant within ~40 minutes. Compared to ultracentrifugation (UC), MS-TFF reduces processing time from ~4 hours to ~40 minutes while achieving a 6-fold higher sEV yield. Clinical validation using urine from 16 subjects (6 healthy/10 prostate cancer patients) enabled quantitative ⁎KLK3/PCA3⁎ RNA biomarker detection, achieving AUC = 0.93 for cancer diagnosis, confirming its diagnostic potential.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.