MiR-155 and MiR-1275 relation with graft-versus-host disease and hepatitis B in hematopoietic stem cell transplant recipients.

Q2 Medicine

VirusDisease Pub Date : 2025-06-01 Epub Date: 2025-08-12 DOI:10.1007/s13337-025-00926-w

Mani Ramzi, Hossain Ali Rostamipour, Mahdiyar Iravani Saadi, Fakhroddin Hosseini, Zahed Karimi, Zahra Shahhossein, Maryam Ahmadyan

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引用次数: 0

Abstract

Introduction: MicroRNAs (miRNAs) play many roles in basic biological processes such as virus proliferation and growth. In this research, we sought to investigate the prognostic and diagnostic potential of miR-155 and miR-1275 in the induction and severity of Graft-Versus-Host Disease (aGVHD) following hematopoietic stem cell transplantation (HSCT). Additionally, we evaluated the association between these miRNAs' expression and HBV infection in patients with HSCT.

Method: In the present research, 135 consecutive patients receiving allogeneic HSCT were enrolled. We assessed the expression levels of miR-155 and miR-1275 in the peripheral blood of patients before and after HSCT by SYBR Green Real-Time PCR. In these patients Hepatitis B virus (HBV) load was also determined by HBV antibody assay.

Results: MiR-1275 and miR-155 expression levels were significantly lower in patients after HSCT. A significant association was found between miR-155 and miR-1275 expression levels in patients who developed aGvHD compared with those without aGvHD. We also found that only MiR-155 expression level was significantly increased in HBV + patients compared with HBV- patients.

Conclusion: Both MiR-1275 and miR-155 genes have the potential to be used as therapeutic targets in HSCT patients in the future. The expression level of both could be employed as biomarkers for aGvHD induction. Moreover, MiR-155 expression level was connected to the pathogenesis of HBV infection.

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造血干细胞移植受者MiR-155和MiR-1275与移植物抗宿主病和乙型肝炎的关系

MicroRNAs （miRNAs）在病毒增殖和生长等基本生物学过程中发挥着重要作用。在这项研究中，我们试图研究miR-155和miR-1275在造血干细胞移植（HSCT）后移植物抗宿主病（aGVHD）的诱导和严重程度中的预后和诊断潜力。此外，我们评估了这些mirna的表达与HSCT患者HBV感染之间的关系。方法：本研究纳入135例连续接受同种异体造血干细胞移植的患者。我们通过SYBR Green Real-Time PCR技术评估了HSCT前后患者外周血中miR-155和miR-1275的表达水平。在这些患者中，乙型肝炎病毒（HBV）载量也通过HBV抗体测定。结果：HSCT后患者的MiR-1275和miR-155表达水平显著降低。研究发现，与没有aGvHD的患者相比，aGvHD患者中miR-155和miR-1275的表达水平显著相关。我们还发现，与HBV-患者相比，HBV +患者中只有MiR-155的表达水平显著升高。结论：MiR-1275和miR-155基因都有可能在未来作为HSCT患者的治疗靶点。两者的表达水平均可作为aGvHD诱导的生物标志物。此外，MiR-155的表达水平与HBV感染的发病机制有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

VirusDisease Medicine-Infectious Diseases

CiteScore

7.00

自引率

0.00%

发文量

期刊介绍： VirusDisease, formerly known as ''Indian Journal of Virology'', publishes original research on all aspects of viruses infecting animal, human, plant, fish and other living organisms.