Molecular characterization of a begomovirus associated with yellow mosaic disease of Acalypha indica.

Q2 Medicine

VirusDisease Pub Date : 2025-06-01 Epub Date: 2025-05-08 DOI:10.1007/s13337-025-00919-9

Sunil Kumar, Deepak Kumar, Olawale Arogundade, Supriya Chakraborty

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Abstract

Begomovirus, a genus within the family Geminiviridae, is the largest genus of plant-infecting viruses. Several recent studies have reported the prevalence of begomoviruses in common weeds. In the present study, Acalypha indica, a euphorbiaceous weed exhibiting yellow mosaic symptoms, was screened for begomovirus infection. The analyzed samples revealed the presence of a bipartite begomovirus with a complete sequence of DNA-A and B components, each of approximately 2.7 kb in size, showing genome organization typical of Old World bipartite begomoviruses. While the DNA-A (GenBank accession number PQ067511) component showed 93% similarity with Jatropha leaf yellow mosaic Katarniaghat virus (JLYMKV-A, JN135236.1), the DNA-B (PQ067512) component closely resembled Rose leaf curl virus (RoLCV-B, OQ440773.1) with approximately 83% similarity. Notably, previous studies have not reported an association of DNA-B components with JLYMKV-A. The sequence analysis revealed that the DNA-B genome has a single recombination event between Sri Lankan cassava mosaic virus DNA-B (SLCMV-B) and RoLCV DNA-B. Further, the common region (CR) analysis revealed a high nucleotide identity of 97% between the DNA-A and B components isolated in this study, suggesting that these are cognate molecules. In summary, the current study, for the first time, reports a novel recombinant DNA-B component with JLYMKV-A causing yellow mosaic disease in A. indica in India.

Supplementary information: The online version contains supplementary material available at 10.1007/s13337-025-00919-9.

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一种与猕猴桃黄花叶病相关的begomvirus的分子特征分析。

Begomovirus是双病毒科的一个属，是最大的植物感染病毒属。最近的几项研究报告了普通杂草中流行的begomovirus。本研究对一种具有黄色花叶症状的大蓬属植物仙人掌进行了begomvirus感染的筛选。分析的样本显示存在一种具有DNA-A和B组分完整序列的双部begomovirus，每个组分的大小约为2.7 kb，显示出典型的东半球双部begomovirus的基因组组织。DNA-A （GenBank登录号PQ067511）成分与麻疯树叶黄花叶Katarniaghat病毒（JLYMKV-A, JN135236.1）相似度为93%，DNA-B （PQ067512）成分与玫瑰叶卷曲病毒（RoLCV-B, OQ440773.1）相似度约为83%。值得注意的是，以前的研究没有报道DNA-B成分与JLYMKV-A的关联。序列分析表明，斯里兰卡木薯花叶病毒DNA-B （SLCMV-B）与斯里兰卡木薯花叶病毒DNA-B之间存在单一重组事件。此外，共同区域（CR）分析显示，在本研究中分离的DNA-A和B组分之间具有97%的高核苷酸同源性，这表明它们是同源分子。综上所述，本研究首次报道了一种新的含有JLYMKV-A的重组DNA-B组分引起印度籼稻黄花叶病。补充信息：在线版本包含补充资料，下载地址为10.1007/s13337-025-00919-9。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

VirusDisease Medicine-Infectious Diseases

CiteScore

7.00

自引率

0.00%

发文量

期刊介绍： VirusDisease, formerly known as ''Indian Journal of Virology'', publishes original research on all aspects of viruses infecting animal, human, plant, fish and other living organisms.