Promoting of Biomechanics' Properties Incisional Wound Repair by Mesenchymal Stem Cell Transplantation.

IF 1 Q3 SURGERY
Maryam Habibi, Jafar Rezaian, Pooria Ali, Rasool Mohammedi, Hassan Ahmadvand, Abolfazl Abaszadeh, Farzaneh Chehelcheraghi
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Abstract

Background: Diabetes mellitus is related to wound healing process impairments and molecular abnormalities of the wound. We aimed to evaluate the therapeutic potential of semi-solid bone marrow as a source of stem cells (SCs) in regenerative medicine.

Methods: This trial study was conducted at the Faculty of Medicine, Lorestan University of Medical Sciences, Khorram Abad, IRAN in 2019. Forty five Albino Wistar rats with an average weight of 250-300g, were purchased from Lorestan Aftabavaran Company in (Khorramabad, Lorestan, Iran). The rats were divided into 3 groups (n=15). For all groups, Streptozotocin (STZ) (Single dosage; 65 mg/kg, i.p.) was used to induce diabetes, and Sharp dissection was used to draw and pull down a dorsal skin flap (9×3 cm) in the control and saline groups on day0, respectively. The group used 7 × 109 BMMSCs on day 0. Histologic specimens were stained with hematoxylin and eosin and were stained with trichrome mason. Biomechanic measurements were taken in the wound area.

Results: The epidermal thickness was increasing and blood vessels were growing. In the cells group, hair follicle destruction, cellular penetration, diffuse fibrosis, and necrosis were not observed in comparison with the saline and control groups. The Cells group had a higher energy absorption than the Bending stiffness and Maximal Force Ultimate Tensile Strength (UTS) group on day 14 as a result of decreased Bending stiffness and Maximal Force Ultimate Tensile Strength (UTS).

Conclusion: According on result of recent study, Stem cells can improve the healing of diabetic incision wounds.

间充质干细胞移植对伤口修复生物力学特性的促进作用。
背景:糖尿病与创面愈合过程障碍和创面分子异常有关。我们旨在评估半固体骨髓作为干细胞(SCs)来源在再生医学中的治疗潜力。方法:本试验研究于2019年在伊朗Khorram Abad的Lorestan医学科学大学医学院进行。45只白化Wistar大鼠,平均体重250-300g,购自伊朗Lorestan Khorramabad的Lorestan Aftabavaran公司。将大鼠分为3组(n=15)。各组均采用链脲佐菌素(STZ)(单次给药,65 mg/kg, ig)诱导糖尿病,对照组和生理盐水组分别于第0天采用Sharp夹层取下背侧皮瓣(9×3 cm)。实验组在第0天使用7 × 109个BMMSCs。组织标本用苏木精和伊红染色,并用三色梅森染色。在创面进行生物力学测量。结果:表皮厚度增加,血管增生。在细胞组中,与生理盐水组和对照组相比,未观察到毛囊破坏、细胞渗透、弥漫性纤维化和坏死。细胞组在第14天的能量吸收高于弯曲刚度组和最大力极限抗拉强度组,这是由于细胞组的弯曲刚度和最大力极限抗拉强度(UTS)降低。结论:根据最近的研究结果,干细胞可以促进糖尿病切口创面的愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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