Han-Heom Na, Hee-Jeong Youk, In Bum Suh, Sang Hoon Lee, Yoonsung Kang, Keun-Cheol Kim
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引用次数: 0
Abstract
Objectives: Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV), a tick-borne pathogen, presents a growing public health threat in East Asia. Although conventional RT-PCR methods are effective for detection, they are limited by the need for specialized equipment and time-consuming procedures. This study aimed to develop and evaluate a rapid, sensitive, and field-deployable diagnostic method for SFTSV using Loop-Mediated Isothermal Amplification (LAMP).
Methods: Twelve sets of LAMP primers were designed to target the RNA-dependent RNA polymerase (L segment) of the SFTSV genome. These primers were screened through stepwise colorimetric LAMP assays to identify the optimal set. The sensitivity and specificity of the selected primer set were evaluated using serial dilutions of SFTSV RNA and a panel of control pathogens.
Results: The selected LAMP primer set demonstrated high amplification efficiency, successfully detecting as little as 10 attograms (ag) of SFTSV RNA. Moreover, the primer set showed no cross-reactivity with non-SFTSV samples, confirming its high specificity.
Conclusion: The developed LAMP assay provides a rapid and reliable method for SFTSV detection, with potential for use in field settings. This diagnostic tool could enhance early detection and improve outbreak response for SFTSV infections.
期刊介绍：
The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.
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