Antibacterial and Antibiofilm Properties of Kulim Bark (Scorodocarpus borneensis Becc.) Hydrogel on Polymicrobial Biofilms.

IF 1.8 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Pharmacopuncture Pub Date : 2025-09-30 DOI:10.3831/KPI.2025.28.3.178

Rafika Sari, Sylvia Utami Tunjung Pratiwi, Yosi Bayu Murti, Ema Damayanti

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引用次数: 0

Abstract

Objectives: The study aimed to analyze the antibacterial and antibiofilm activity of hydrogels from Kulim (Scorodocarpus borneensis Becc.) stem bark extract and fraction against polymicrobial biofilms.

Methods: The stem bark extract and fraction of Kulim (Scorodocarpus borneensis Becc.) were formulated into a hydrogel (tween 80glycerin). Antibacterial and antibiofilm activities were assessed using a microdilution test against polymicrobial growth and polymicrobial biofilms, while acute toxicity was evaluated using wader pari (Rasbora lateristriata) embryos.

Results: Kulim extract hydrogel (4 μg/µL) showed 69.16% antibacterial activity and 61.25% biofilm inhibition, with 72.58% and 82.86% biofilm degradation at 24 and 48 hours, respectively. The fraction-based hydrogel (0.22 μg/µL) had 58.10% antibacterial and biofilm inhibition activity, with 81.14% and 79.64% degradation at 24 and 48 hours. MIC50 and MBIC50 values were 2.42 μg/µL (extract) and 0.15 μg/µL (fraction) as well as 2.83 μg/µL and 0.19 μg/µL, respectively. Based on observation, MBEC50 values were 1.09 μg/µL and 0.57 μg/µL (24 h), as well as 0.70 μg/µL and 0.03 μg/µL (48 h), respectively, while the extract's LC50 was 0.143 mg/mL.

Conclusion: The hydrogel made from Kulim stem bark extract exhibited stronger antibacterial and antibiofilm activities than the ethyl acetate fraction against polymicrobial.

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竹林树皮的抗菌和抗生物膜性能多微生物生物膜上的水凝胶。

目的：研究龙骨茎皮提取物及部位水凝胶对多种微生物生物膜的抑菌和抑膜活性。方法：将苦参茎皮提取液和部分配制成水凝胶（甘油为80）。采用微稀释试验对多微生物生长和多微生物生物膜进行抑菌和抗生物膜活性评估，并使用红颊鼠（Rasbora lateristriata）胚胎评估急性毒性。结果：苦林提取物水凝胶（4 μg/µL）的抑菌活性为69.16%，生物膜抑制率为61.25%，24 h和48 h生物膜降解率分别为72.58%和82.86%。0.22 μg/µL的水凝胶具有58.10%的抗菌和生物膜抑制活性，24和48 h的降解率分别为81.14%和79.64%。MIC50和MBIC50分别为2.42 μg/µL（提取物）和0.15 μg/µL（馏分），2.83 μg/µL和0.19 μg/µL。经观察，MBEC50值分别为1.09、0.57 μg/µL （24 h）、0.70、0.03 μg/µL (48 h)， LC50值为0.143 mg/mL。结论：榆木茎皮提取物水凝胶对多种微生物的抑菌和抗膜活性优于乙酸乙酯部分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacopuncture INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.10

自引率

7.10%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.