Comparison of the effects of continuous and pulsed blue light for reduction of Enterococcus faecalis biofilms.

IF 2.4 4区医学 Q3 ENGINEERING, BIOMEDICAL

Lasers in Medical Science Pub Date : 2025-09-29 DOI:10.1007/s10103-025-04663-w

Emad Kosarieh, Sahar Sattari Khavas, Nasim Chiniforush, Shima Afrasiabi

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引用次数: 0

Abstract

Mature biofilms exhibited minimal antimicrobial susceptibility. This study compares the decontamination effect of 450 nm continuous wave (CW) and pulsed wave (PW) blue diode laser with two different photosensitizers (curcumin and riboflavin) against mature Enterococcus faecalis biofilm model. Four irradiation parameter sets A (CW, peak power 0.4 W, and average fluence of 24 J/cm²), B (PW, duty cycle (DC) 50%, peak power 0.8 W, and average fluence of 24 J/cm²), C (CW, peak power 0.5 W, and average fluence of 30 J/cm²), and D (PW, DC 50%, peak power 1.0 W, and average fluence of 30 J/cm²) were used. The results showed that all irradiation parameters achieved a significant bacterial reduction compared to the control (p < 0.001). However, PW showed a more pronounced reduction effect than CW, with the difference being particularly marked at an average fluence of 30 J/cm². Curcumin or riboflavin without 450 nm blue diode laser irradiation had no antimicrobial effect (p > 0.05). PW light has the potential to improve the efficacy of a blue diode laser. However, the correct choice of parameters is required to achieve adequate laser fluence.

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连续蓝光与脉冲蓝光对粪肠球菌生物膜还原效果的比较。

成熟的生物膜表现出最小的抗菌敏感性。本研究比较了450 nm连续波（CW）和脉冲波（PW）蓝色二极管激光在姜黄素和核黄素两种光敏剂作用下对成熟粪肠球菌生物膜模型的去污效果。采用4个辐照参数集A（连续波，峰值功率0.4 W，平均影响24 J/cm2）、B （PW，占空比50%，峰值功率0.8 W，平均影响24 J/cm2）、C（连续波，峰值功率0.5 W，平均影响30 J/cm2）和D （PW, DC 50%，峰值功率1.0 W，平均影响30 J/cm2）。结果表明，与对照组相比，所有辐照参数均能显著减少细菌（p 2）。姜黄素和核黄素未经450 nm蓝光二极管激光照射后无抗菌作用（p < 0.05）。PW光有可能提高蓝色二极管激光器的效率。然而，要获得足够的激光通量，需要正确选择参数。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lasers in Medical Science 医学-工程：生物医学

CiteScore

4.50

自引率

4.80%

发文量

192

审稿时长

3-8 weeks

期刊介绍： Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.