{"title":"The simultaneous protective effect of photobiomodulation on root resorption while accelerating orthodontic tooth movement in rats.","authors":"Lin Kong, Ke Zhang, Fei Liu, Ying Zhao","doi":"10.1007/s10103-025-04652-z","DOIUrl":null,"url":null,"abstract":"<p><p>To investigate the effect of photobiomodulation (PBM) on orthodontically induced root resorption (OIRR) while accelerating orthodontic tooth movement (OTM), and explore the possible role of periodontal ligament stem cells (PDLSCs) during this process. Thirty rats were randomly divided into 5 groups (A1-A5). The right upper first molars (UM1s) were assigned as PBM side (P), receiving different energy densities of PBM: A1-P (3.3 J/cm<sup>2</sup>); A2-P (5 J/cm<sup>2</sup>); A3-P (6.7 J/cm<sup>2</sup>); A4-P (8.3 J/cm<sup>2</sup>); A5-P (10 J/cm<sup>2</sup>). A diode laser (650 nm, 30 mW) with 0.09 cm<sup>2</sup> beam area was used to irradiate right UM1s at three points (the mesial, buccal and palatal sides) over the root and the irradiation time per point was 10 s in group A1, 15 s in group A2, 20 s in group A3, 25 s in group A4 and 30 s in group A5. The left UM1s were split-mouth control side. Bilateral UM1s were moved mesially by 50 g force for 14 days. The amount of OTM, root length (RL), total root resorption volume (T-RRV), RRV of compression zone (RRV-CZ) and RRV of tension zone (RRV-TZ) were evaluated by micro-CT, and CAP expression level of PDLSCs was analyzed by IHC staining. Groups of 5 and 6.7 J/cm<sup>2</sup> achieved significant accelerated orthodontic tooth movement (AOTM). There was no significant difference of RL, T-RRV and RRV-CZ between PBM and control sides. PBM irradiation at 5-10 J/cm<sup>2</sup> significantly reduced RRV-TZ. Moreover, the 6.7 J/cm<sup>2</sup> group achieved the largest AOTM efficiency and showed the highest reduction rate in decreasing RRV-TZ. The expression level of CAP was significantly higher on PBM side than that on control side in both compression and tension zones. Diode laser (650 nm, 30 mW) of 5-10 J/cm<sup>2</sup> had a protective effect on orthodontic tooth root by upregulating the cementoblastic differentiation of PDLSCs. Particularly, diode laser of 6.7 J/cm<sup>2</sup> exhibited the best efficiency in preventing OIRR of tension zone while accelerating OTM.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"394"},"PeriodicalIF":2.4000,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lasers in Medical Science","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10103-025-04652-z","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
Abstract
To investigate the effect of photobiomodulation (PBM) on orthodontically induced root resorption (OIRR) while accelerating orthodontic tooth movement (OTM), and explore the possible role of periodontal ligament stem cells (PDLSCs) during this process. Thirty rats were randomly divided into 5 groups (A1-A5). The right upper first molars (UM1s) were assigned as PBM side (P), receiving different energy densities of PBM: A1-P (3.3 J/cm2); A2-P (5 J/cm2); A3-P (6.7 J/cm2); A4-P (8.3 J/cm2); A5-P (10 J/cm2). A diode laser (650 nm, 30 mW) with 0.09 cm2 beam area was used to irradiate right UM1s at three points (the mesial, buccal and palatal sides) over the root and the irradiation time per point was 10 s in group A1, 15 s in group A2, 20 s in group A3, 25 s in group A4 and 30 s in group A5. The left UM1s were split-mouth control side. Bilateral UM1s were moved mesially by 50 g force for 14 days. The amount of OTM, root length (RL), total root resorption volume (T-RRV), RRV of compression zone (RRV-CZ) and RRV of tension zone (RRV-TZ) were evaluated by micro-CT, and CAP expression level of PDLSCs was analyzed by IHC staining. Groups of 5 and 6.7 J/cm2 achieved significant accelerated orthodontic tooth movement (AOTM). There was no significant difference of RL, T-RRV and RRV-CZ between PBM and control sides. PBM irradiation at 5-10 J/cm2 significantly reduced RRV-TZ. Moreover, the 6.7 J/cm2 group achieved the largest AOTM efficiency and showed the highest reduction rate in decreasing RRV-TZ. The expression level of CAP was significantly higher on PBM side than that on control side in both compression and tension zones. Diode laser (650 nm, 30 mW) of 5-10 J/cm2 had a protective effect on orthodontic tooth root by upregulating the cementoblastic differentiation of PDLSCs. Particularly, diode laser of 6.7 J/cm2 exhibited the best efficiency in preventing OIRR of tension zone while accelerating OTM.
期刊介绍：
Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics.
The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.
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