The Association of VDR FokI T>C (rs2228570) Gene Variants With T2DM, and Its Complications: Influence on BMI, Oxidative Stress, and Dyslipidemia

IF 2.9 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-09-29 DOI:10.1002/jcla.70090

Armin Sharifi, Mahsa Nouri, Ebrahim Shakiba, Zahra Ghorbani, Zohreh Rahimi

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Abstract

Introduction

The present study aimed to investigate the association between vitamin D receptor (VDR) FokI gene variants and the risk of type 2 diabetes mellitus (T2DM) and its complications, as well as with oxidative stress parameters.

Methods

We investigated 300 individuals with diabetes with and without neuropathy and retinopathy, and 100 individuals without diabetes. The PCR-RFLP technique was used to determine the genotypes of VDR FokI T>C (rs2228570). The oxidative stress parameters were measured using chemical methods.

Results

In individuals with diabetes, there were significantly higher levels of body mass index (BMI) and oxidative stress than in controls. The presence of the FokI CC genotype and the C allele were associated with 2.46 times and 1.6-fold increased risk of T2DM, respectively, and enhanced diabetic neuropathy risk by 3.41- and 3.68-fold, respectively, and elevated diabetic retinopathy risk by 2.45 and 1.59 times, respectively. The presence of FokI TC + CC compared to the TT genotype resulted in higher triglycerides, total oxidative status, and BMI levels in individuals with diabetes. We found significantly higher BMI, oxidative stress index, and significantly lower levels of total antioxidant capacity in females than in males among individuals with diabetes and controls.

Conclusion

This study indicates that the FokI CC genotype and the FokI C allele are associated with an increased risk of T2DM and its complications. We observed the influence of VDR FokI variants on dyslipidemia, oxidative stress, and BMI. It seems the risk factors of developing T2DM, obesity, and oxidative stress among women are more prevalent than in men.

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VDR FokI t> C （rs2228570）基因变异与T2DM及其并发症的关系：对BMI、氧化应激和血脂异常的影响

本研究旨在探讨维生素D受体（VDR） FokI基因变异与2型糖尿病（T2DM）及其并发症的风险以及氧化应激参数之间的关系。方法：我们调查了300例伴有或不伴有神经病变和视网膜病变的糖尿病患者和100例无糖尿病患者。采用PCR-RFLP技术对VDR FokI T>C （rs2228570）进行基因型鉴定。采用化学方法测定氧化应激参数。结果：糖尿病患者的身体质量指数（BMI）和氧化应激水平明显高于对照组。FokI CC基因型和C等位基因的存在与T2DM风险分别增加2.46倍和1.6倍，糖尿病神经病变风险分别增加3.41倍和3.68倍，糖尿病视网膜病变风险分别增加2.45倍和1.59倍相关。与TT基因型相比，FokI TC + CC的存在导致糖尿病患者甘油三酯、总氧化状态和BMI水平升高。我们发现，在糖尿病患者和对照组中，女性的BMI和氧化应激指数明显高于男性，而总抗氧化能力水平明显低于男性。结论：本研究提示FokI CC基因型和FokI C等位基因与T2DM及其并发症风险增加相关。我们观察了VDR FokI变异对血脂异常、氧化应激和BMI的影响。女性患2型糖尿病、肥胖和氧化应激的风险因素似乎比男性更普遍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.