Coexistence of Multidrug Resistance and Hypervirulence-Associated Genes in Clinical Carbapenem-Resistant Pseudomonas aeruginosa.

IF 3.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hye Hyun Cho, Yumi Park
{"title":"Coexistence of Multidrug Resistance and Hypervirulence-Associated Genes in Clinical Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>.","authors":"Hye Hyun Cho, Yumi Park","doi":"10.4014/jmb.2505.05031","DOIUrl":null,"url":null,"abstract":"<p><p>The recent worldwide emergence of hypervirulent, multidrug-resistant <i>Pseudomonas aeruginosa</i> represents a critical public health threat. The molecular typing, multidrug resistance (MDR) rates, and hypervirulence-associated genes in carbapenem-resistant <i>P. aeruginosa</i> (CRPA) isolates can vary across geographic locations and patients, highlighting their clinical significance. In this study we investigated the epidemiology and relationship between MDR and the presence of hypervirulence-associated genes in CRPA isolates. Accordingly, we performed antimicrobial susceptibility tests, multilocus sequence typing (MLST), and PCR-based detection of carbapenemase and virulence-associated genes. Notably, 34.9% (139/398) of the isolates were carbapenem-resistant, with 79.1% of these exhibiting MDR. Of the 30 sequence types (STs) identified by MLST, ST773 was the most prevalent (28.8%), followed by ST235 (23.0%). New Delhi metallo-β-lactamase (NDM)-1 or imipenemase-6 occurred in 45.3% of the CRPA strains. Common virulence genes included <i>exoT</i> (100.0% of isolates), <i>exoY</i> (95.7%), <i>exoU</i> (66.2%), and <i>exoS</i> (34.5%). <i>exoU</i> was associated with antibiotic resistance (<i>p</i><0.05), except for carbapenems in CRPA isolates. The <i>exoU</i>/<i>exoT</i>/<i>exoY</i> Type III secretion system (T3SS) genotype was the most prevalent among the carbapenemase-producing CRPA strains. Among the 139 CRPA isolates, we identified a pandrug-resistant, NDM-1-producing ST235 strain co-expressing <i>exoS</i> and <i>exoU</i>. This study highlights the important association between <i>exoU</i> and MDR, indicating a potential relationship between T3SS genotypes and antibiotic resistance. Additionally, the identification of a hypervirulent, pandrug-resistant <i>P. aeruginosa</i> ST235 isolate harboring <i>bla</i><sub>IMP-6</sub> underscores the critical need for enhanced surveillance against high-risk strains.</p>","PeriodicalId":16481,"journal":{"name":"Journal of microbiology and biotechnology","volume":"35 ","pages":"e2505031"},"PeriodicalIF":3.1000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4014/jmb.2505.05031","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The recent worldwide emergence of hypervirulent, multidrug-resistant Pseudomonas aeruginosa represents a critical public health threat. The molecular typing, multidrug resistance (MDR) rates, and hypervirulence-associated genes in carbapenem-resistant P. aeruginosa (CRPA) isolates can vary across geographic locations and patients, highlighting their clinical significance. In this study we investigated the epidemiology and relationship between MDR and the presence of hypervirulence-associated genes in CRPA isolates. Accordingly, we performed antimicrobial susceptibility tests, multilocus sequence typing (MLST), and PCR-based detection of carbapenemase and virulence-associated genes. Notably, 34.9% (139/398) of the isolates were carbapenem-resistant, with 79.1% of these exhibiting MDR. Of the 30 sequence types (STs) identified by MLST, ST773 was the most prevalent (28.8%), followed by ST235 (23.0%). New Delhi metallo-β-lactamase (NDM)-1 or imipenemase-6 occurred in 45.3% of the CRPA strains. Common virulence genes included exoT (100.0% of isolates), exoY (95.7%), exoU (66.2%), and exoS (34.5%). exoU was associated with antibiotic resistance (p<0.05), except for carbapenems in CRPA isolates. The exoU/exoT/exoY Type III secretion system (T3SS) genotype was the most prevalent among the carbapenemase-producing CRPA strains. Among the 139 CRPA isolates, we identified a pandrug-resistant, NDM-1-producing ST235 strain co-expressing exoS and exoU. This study highlights the important association between exoU and MDR, indicating a potential relationship between T3SS genotypes and antibiotic resistance. Additionally, the identification of a hypervirulent, pandrug-resistant P. aeruginosa ST235 isolate harboring blaIMP-6 underscores the critical need for enhanced surveillance against high-risk strains.

耐碳青霉烯铜绿假单胞菌多药耐药与高毒力相关基因共存的研究
最近在世界范围内出现的高毒力、耐多药铜绿假单胞菌构成了严重的公共卫生威胁。耐碳青霉烯P. aeruginosa (CRPA)分离株的分子分型、多药耐药(MDR)率和高毒力相关基因可能因地理位置和患者而异,这突出了它们的临床意义。在这项研究中,我们调查了CRPA分离株中MDR与高毒力相关基因存在的流行病学和关系。因此,我们进行了抗菌药敏试验、多位点序列分型(MLST)和基于pcr的碳青霉烯酶和毒力相关基因检测。值得注意的是,34.9%(139/398)的分离株对碳青霉烯耐药,其中79.1%表现为耐多药。在MLST鉴定的30种序列类型(STs)中,以ST773最为常见(28.8%),其次是ST235(23.0%)。45.3%的CRPA菌株中存在新德里金属β-内酰胺酶(NDM)-1或亚胺烯酶-6。常见的毒力基因包括exoT(100.0%)、exy(95.7%)、exoU(66.2%)和exoS(34.5%)。exoU与抗生素耐药性相关(peexou /exoT/exoY III型分泌系统(T3SS)基因型在产碳青霉烯酶的CRPA菌株中最为普遍)。在139株CRPA分离株中,我们鉴定出一株具有大范围耐药、产生ndm -1的ST235菌株,该菌株共表达exo和exoU。该研究强调了exoU与MDR之间的重要关联,表明T3SS基因型与抗生素耐药性之间存在潜在关系。此外,一株携带blaIMP-6的高毒力、耐药铜绿假单胞菌ST235分离株的鉴定强调了加强对高风险菌株监测的迫切需要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信