Spatial and temporal mapping of early alphaherpesvirus invasion routes into the mouse central nervous system.

IF 1.9 4区 医学 Q3 NEUROSCIENCES
Viktoria Korff, Issam El-Debs, Julia Sehl-Ewert
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引用次数: 0

Abstract

Alphaherpesviruses such as Herpes Simplex Virus 1 (HSV-1) and Pseudorabies virus (PrV) invade the central nervous system (CNS) via peripheral nerves. While olfactory and trigeminal pathways are well-known, additional cranial routes remain underexplored. Using a PrV-∆UL21gfp/US3∆kin mutant in CD1 mice, we mapped early neuroinvasion (4-96 hpi) by immunofluorescence and RNA in situ hybridization. Viral antigen and lytic viral gene expression (UL19 RNA) were detected in the olfactory epithelium, vomeronasal organ, incisors, palate, olfactory bulb, and brainstem. These results indicate multineural CNS access involving olfactory (I), trigeminal (V), glossopharyngeal (IX), and hypoglossal (XII) nerves, highlighting this model's value for studying early alphaherpesvirus spread.

早期甲疱疹病毒侵入小鼠中枢神经系统的时空图谱。
甲型疱疹病毒如单纯疱疹病毒1型(HSV-1)和伪狂犬病毒(PrV)通过周围神经侵入中枢神经系统(CNS)。虽然嗅觉和三叉神经通路是众所周知的,但其他的颅脑通路仍未被充分探索。利用CD1小鼠的PrV-∆UL21gfp/US3∆kin突变体,我们通过免疫荧光和RNA原位杂交绘制了早期神经侵袭(4-96 hpi)。在嗅上皮、犁头鼻器官、门牙、上颚、嗅球和脑干中检测到病毒抗原和溶酶病毒基因表达(UL19 RNA)。这些结果表明,涉及嗅觉(I)、三叉神经(V)、舌咽神经(IX)和舌下神经(XII)的多神经中枢通路,突出了该模型在研究早期甲疱疹病毒传播方面的价值。
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来源期刊
Journal of NeuroVirology
Journal of NeuroVirology 医学-病毒学
CiteScore
6.60
自引率
3.10%
发文量
77
审稿时长
6-12 weeks
期刊介绍: The Journal of NeuroVirology (JNV) provides a unique platform for the publication of high-quality basic science and clinical studies on the molecular biology and pathogenesis of viral infections of the nervous system, and for reporting on the development of novel therapeutic strategies using neurotropic viral vectors. The Journal also emphasizes publication of non-viral infections that affect the central nervous system. The Journal publishes original research articles, reviews, case reports, coverage of various scientific meetings, along with supplements and special issues on selected subjects. The Journal is currently accepting submissions of original work from the following basic and clinical research areas: Aging & Neurodegeneration, Apoptosis, CNS Signal Transduction, Emerging CNS Infections, Molecular Virology, Neural-Immune Interaction, Novel Diagnostics, Novel Therapeutics, Stem Cell Biology, Transmissable Encephalopathies/Prion, Vaccine Development, Viral Genomics, Viral Neurooncology, Viral Neurochemistry, Viral Neuroimmunology, Viral Neuropharmacology.
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