Effects of plant-based fermented broth on rumen fermentation, gastrointestinal development, and microbial populations in fattening lambs.

IF 2.9 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1584930

Lu Zhang, Huiwen Zhang, Lianjie Song, Yongliang Li, Jianjun Guo, Feng Li, Bosen Li, Wei Chen, Yuqing Liu, Haitong Yang, Jianjie Li, Yuhong Gao, Xinsheng Sun

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引用次数: 0

Abstract

Introduction: Digestive dysfunction in lamb fattening has been a concern in recent years. The objective of this study was to investigate the effects of a fermentation broth (FB) derived from eight plants on rumen fermentation, gastrointestinal development, and microbial community composition in fattening lambs through in vitro and in vivo experiments.

Methods: In vitro rumen fermentation was performed with six FB: diet ratios (mL/g): 0, 1:125, 1:250, 1:500, 1:1000, and 1:2000. Fermentation parameters and nutrient disappearance rates were measured over 48 h. Based on the results of the in vitro test, two optimal doses of FB (1:500 and 1:1000) were selected for further in vivo testing over a 120-day period. A total of 90 weaned small-tailed Han lambs were assigned to three groups, receiving FB in drinking water at 0 (control), 1:500, or 1:1000 (vol/vol).

Results: The results showed that three parameters of gas production (GP), including fast-degradable fraction, slow-degradable fraction, and theoretical GP, were significantly higher (p < 0.05) in the 1:500 and 1:1000 groups compared with other groups. In vivo tests further showed that FB supplementation at 1:500 and 1:1000 improved rumen microstructure (papilla length, papilla surface, mucosa thickness, and muscle thickness) and jejunal microstructure (villus height, villus surface, and muscle thickness). Moreover, two rumen bacterial indices, PD_whole_tree (p = 0.06) and Shannon (p = 0.07), demonstrated increasing trends in both FB groups relative to the control group. In the rumen, 1:500 FB supplementation increased the abundance of Firmicutes (p < 0.01) and decreased Bacteroidota abundance (p < 0.01). In the jejunum, 1:500 FB supplementation decreased the abundance of Euryarchaeota and Methanobrevibacter (both p = 0.02) while increasing the abundance of Actinomycetes and Aeriscardovia (both p = 0.01) compared to the control.

Discussion: In summary, FB supplementation in lambs' drinking water at a ratio of 1:500 (FB: diet, mL/g) improved rumen fermentation and promoted microecological balance.

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植物发酵肉汤对育肥羔羊瘤胃发酵、胃肠道发育和微生物种群的影响。

近年来，羔羊育肥过程中的消化功能障碍一直是人们关注的问题。本试验旨在通过体外和体内试验，研究8种植物发酵液对育肥羔羊瘤胃发酵、胃肠道发育和微生物群落组成的影响。方法：采用酵母与饲料（mL/g）的比例（mL/g）分别为0、1:125、1:250、1:500、1:1000和1:2000进行体外瘤胃发酵。在48 h内测定发酵参数和营养物质消失率。根据体外试验结果，选择两种最佳剂量（1:500和1:100）进行120天的体内试验。试验选用90只断奶小尾汉族羔羊，分为3组，分别在0（对照）、1:500和1:100 （vol/vol）的水中添加FB。结果：结果表明，快速降解分数、慢降解分数和理论产气量三个参数显著提高（p ），体内试验进一步表明，在1:500和1:100添加FB改善了瘤胃微观结构（乳头长度、乳头表面、黏膜厚度和肌肉厚度）和空肠微观结构（绒毛高度、绒毛表面和肌肉厚度）。此外，两组瘤胃细菌指数PD_whole_tree （p = 0.06）和Shannon （p = 0.07）均较对照组升高。在瘤胃中，与对照组相比，添加1:500 FB增加了厚壁菌门（p p ）的丰度（p = 0.02），增加了放线菌和无氧菌（p = 0.01）的丰度（p = 0.02）。综上所述，在羔羊饮水中以1:500的比例添加FB (FB：日粮，mL/g)，改善了瘤胃发酵，促进了微生态平衡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.