6-gingerol promotes apoptosis of ovarian cancer cells through miR-506/Gli3 signaling pathway activation.

IF 3.5 3区医学 Q2 ONCOLOGY

Frontiers in Oncology Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI:10.3389/fonc.2025.1547771

Jun Xiong, Hong-Hu Wu, Hui Jiang, Huan Li, Xiao-Qing Tan, Xiao-Ju He, Xue-Xin Cheng

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引用次数: 0

Abstract

Purpose: Ginger rhizomes have shown potential for promoting human health, including the prevention and treatment of cancer. Here, we investigated the anticancer activities of 6-gingerol and explored its mechanisms of action in ovarian cancer cells.

Methods: SKOV3 ovarian cancer cells were treated with different concentrations of 6-gingerol. Clonogenic assays, Flow cytometry, and Western blotting were used to evaluate cell survival and apoptosis. RT-qPCR and transfection experiments were performed to assess the role of miR-506, and bioinformatics tools were used to identify Gli3 as a target gene.

Results: In vitro, ovarian cancer cells underwent apoptosis following 6-gingerol treatment. 6-Gingerol suppressed Gli3 expression without affecting Bax, Bcl-2, or Bcl-xL levels. Low miR-506 expression was observed in ovarian cancer tissues, whereas 6-gingerol significantly promoted its expression. miR-506 directly suppressed Gli3 expression and induced apoptosis in SKOV3 cells.

Conclusions: Our results indicate that gingerol promoted the upregulation of miR-506, leading to the induction of apoptosis in ovarian cancer cells. This study supports the potential of 6-gingerol-based therapy for ovarian malignancies.

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6-姜辣素通过激活miR-506/Gli3信号通路促进卵巢癌细胞凋亡。

目的：生姜根茎具有促进人体健康的潜力，包括预防和治疗癌症。本文对6-姜辣素的抗肿瘤活性进行了研究，并探讨了其对卵巢癌细胞的作用机制。方法：用不同浓度的6-姜辣素处理SKOV3卵巢癌细胞。克隆源实验、流式细胞术和Western blotting检测细胞存活和凋亡情况。通过RT-qPCR和转染实验来评估miR-506的作用，并使用生物信息学工具鉴定Gli3为靶基因。结果：6-姜辣素处理后，卵巢癌细胞在体外发生凋亡。6-姜辣素抑制Gli3的表达，而不影响Bax、Bcl-2或Bcl-xL的水平。miR-506在卵巢癌组织中低表达，而6-姜辣素显著促进其表达。miR-506直接抑制SKOV3细胞Gli3的表达，诱导细胞凋亡。结论：我们的研究结果表明姜辣素可促进miR-506的上调，从而诱导卵巢癌细胞凋亡。这项研究支持了6-姜辣素治疗卵巢恶性肿瘤的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Oncology Biochemistry, Genetics and Molecular Biology-Cancer Research

CiteScore

6.20

自引率

10.60%

发文量

6641

审稿时长

14 weeks

期刊介绍： Cancer Imaging and Diagnosis is dedicated to the publication of results from clinical and research studies applied to cancer diagnosis and treatment. The section aims to publish studies from the entire field of cancer imaging: results from routine use of clinical imaging in both radiology and nuclear medicine, results from clinical trials, experimental molecular imaging in humans and small animals, research on new contrast agents in CT, MRI, ultrasound, publication of new technical applications and processing algorithms to improve the standardization of quantitative imaging and image guided interventions for the diagnosis and treatment of cancer.