Identification and characterization of salivary immune complex antigens in oral cancer.

IF 3.1 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Investigations Pub Date : 2025-09-29 DOI:10.1007/s00784-025-06549-1

Maria Sei, Sayo Kanda, Nozomi Aibara, Rean Hashimura, Junya Hashizume, Yuki Jimbayashi Kutsuna, Tomofumi Naruse, Keisuke Omori, Hironori Sakai, Shin-Ichi Yamada, Takumi Hasegawa, Daisuke Takeda, Nobuhiro Yamakawa, Tadaaki Kirita, Tomoaki Kurosaki, Hirotaka Miyamoto, Kayoko Sato, Yukinobu Kodama, Mihoko N Nakashima, Mikiro Nakashima, Souichi Yanamoto, Kaname Ohyama

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引用次数: 0

Abstract

Objectives: This study aims to identify antigens incorporated into immune complexes that are associated with oral cancer pathogenesis and to determine their potential clinical applications.

Materials and methods: In this study, immune complexome analysis was performed on saliva samples from 66 patients with oral cancer and 40 controls. Immune complexome analysis is an original method for the comprehensive identification of immune complex antigens in body fluids by liquid chromatography-mass spectrometry.

Results: We identified 24 IC-antigens that were significantly elevated in early-stage oral cancer compared with the controls, suggesting their potential as indicators for oral cancer. Four IC-antigens (ALOX5, B3GALT6, LAMA4, POLA1) were expressed at significantly higher levels in advanced-stage disease, indicating their involvement in cancer progression. Survival analysis revealed that high levels of POLA1 were associated with lower survival rates. Most of these proteins were reported to be involved in cancer progression and cell proliferation.

Conclusion: These findings support the formation of disease-characteristic immune complexes in oral cancer and highlight the potential of immune complexome analysis in saliva for understanding oral cancer pathogenesis and therapeutic targets.

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口腔癌中唾液免疫复合物抗原的鉴定与表征。

目的：本研究旨在鉴定与口腔癌发病机制相关的免疫复合物抗原，并确定其潜在的临床应用。材料和方法：本研究对66例口腔癌患者和40例对照组的唾液样本进行免疫复合物组分析。免疫复合物分析是液相色谱-质谱联用技术对体液免疫复合物抗原进行综合鉴定的一种新颖方法。结果：与对照组相比，我们鉴定出24种ic抗原在早期口腔癌中显著升高，这表明它们有可能作为口腔癌的指标。四种ic抗原（ALOX5, B3GALT6, LAMA4, POLA1）在晚期疾病中表达水平显著升高，表明它们参与癌症进展。生存分析显示，高水平的POLA1与较低的存活率相关。据报道，这些蛋白质中的大多数与癌症进展和细胞增殖有关。结论：这些发现支持口腔癌中疾病特征免疫复合物的形成，并突出了唾液免疫复合物组分析在了解口腔癌发病机制和治疗靶点方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Oral Investigations 医学-牙科与口腔外科

CiteScore

6.30

自引率

5.90%

发文量

484

审稿时长

3 months

期刊介绍： The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.