SIRT1 Expression in Human Gastrointestinal Tumors and Its Clinical Significance

IF 3.1 2区医学 Q2 ONCOLOGY

Cancer Medicine Pub Date : 2025-09-29 DOI:10.1002/cam4.71217

Wenxuan Liu, Xinyi Li, Wenhong Deng

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引用次数: 0

Abstract

Objective

This study aimed to investigate the expression levels of SIRT1 protein in gastric (GC), colon (CC), and rectal cancer (RC) tissues and patient plasma, analyze its correlation with clinicopathological features and prognosis, and preliminarily explore its relationship with the tumor immune microenvironment.

Methods

Plasma samples were collected from 198 gastrointestinal cancer patients (66 each of GC, CC, and RC) and 66 healthy volunteers. Additionally, cancerous and adjacent normal tissues were obtained from 45 of these patients (15 pairs for each cancer type). SIRT1 concentration in plasma was detected using Enzyme-Linked Immunosorbent Assay (ELISA). SIRT1 protein expression in tissues was examined using Immunohistochemistry (IHC) and Western Blotting. Bioinformatic analysis was performed using TCGA and GEPIA databases. The correlation between SIRT1 and immune cell infiltration was analyzed via the TIMER database. Statistical analyses were conducted using SPSS software.

Results

Plasma SIRT1 concentration was significantly higher in the GC group compared to healthy controls (p = 0.045), while it was significantly lower in the CC and RC groups (p = 0.007 and p = 0.009, respectively). In tissues, SIRT1 expression was up-regulated in GC but down-regulated in colorectal cancer (CC and RC) tissues (p < 0.05). SIRT1 expression levels were significantly correlated with clinicopathological features including tumor differentiation degree, depth of invasion, TNM stage, distant metastasis (in colorectal cancer), and lymph node metastasis (in RC) (p < 0.05). Survival analysis revealed that high SIRT1 expression was associated with poorer overall survival (OS) in GC patients (p = 0.032), while low expression was associated with poorer OS in RC patients (p = 0.027). Plasma SIRT1 levels showed significant correlations with various tumor markers (e.g., CEA, CA199, CA125, CA724). Furthermore, SIRT1 expression was positively correlated with the infiltration levels of immune cells such as CD4+ T cells, CD8+ T cells, macrophages, and neutrophils.

Conclusion

SIRT1 expression in gastrointestinal tumors is tissue-specific (upregulated in GC, downregulated in colorectal cancer). Its expression level is closely associated with malignant progression and patient prognosis, and it may be involved in the modulation of the tumor immune microenvironment. SIRT1 shows promise as a potential diagnostic biomarker and therapeutic target for gastrointestinal cancers.

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SIRT1在人胃肠道肿瘤中的表达及其临床意义

目的：本研究旨在探讨SIRT1蛋白在胃癌（GC）、结肠癌（CC）、直肠癌（RC）组织及患者血浆中的表达水平，分析其与临床病理特征及预后的相关性，并初步探讨其与肿瘤免疫微环境的关系。方法：收集198例胃肠道肿瘤患者（GC、CC、RC各66例）和66名健康志愿者的血浆样本。此外，从45名患者（每种癌症类型15对）中获得癌组织和邻近正常组织。采用酶联免疫吸附试验（ELISA）检测血浆中SIRT1浓度。采用免疫组化（IHC）和Western Blotting检测组织中SIRT1蛋白的表达。采用TCGA和GEPIA数据库进行生物信息学分析。通过TIMER数据库分析SIRT1与免疫细胞浸润的相关性。采用SPSS软件进行统计分析。结果：GC组血浆SIRT1浓度显著高于健康对照组（p = 0.045），而CC组和RC组血浆SIRT1浓度显著低于健康对照组（p = 0.007和p = 0.009）。在组织中，SIRT1在胃癌中表达上调，而在结直肠癌（CC和RC）组织中表达下调(p结论：SIRT1在胃肠道肿瘤中的表达具有组织特异性（胃癌中表达上调，结直肠癌中表达下调）。其表达水平与恶性进展和患者预后密切相关，并可能参与肿瘤免疫微环境的调节。SIRT1有望成为胃肠道癌症的潜在诊断生物标志物和治疗靶点。

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来源期刊

Cancer Medicine ONCOLOGY-

CiteScore

5.50

自引率

2.50%

发文量

907

审稿时长

19 weeks

期刊介绍： Cancer Medicine is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research from global biomedical researchers across the cancer sciences. The journal will consider submissions from all oncologic specialties, including, but not limited to, the following areas: Clinical Cancer Research Translational research ∙ clinical trials ∙ chemotherapy ∙ radiation therapy ∙ surgical therapy ∙ clinical observations ∙ clinical guidelines ∙ genetic consultation ∙ ethical considerations Cancer Biology: Molecular biology ∙ cellular biology ∙ molecular genetics ∙ genomics ∙ immunology ∙ epigenetics ∙ metabolic studies ∙ proteomics ∙ cytopathology ∙ carcinogenesis ∙ drug discovery and delivery. Cancer Prevention: Behavioral science ∙ psychosocial studies ∙ screening ∙ nutrition ∙ epidemiology and prevention ∙ community outreach. Bioinformatics: Gene expressions profiles ∙ gene regulation networks ∙ genome bioinformatics ∙ pathwayanalysis ∙ prognostic biomarkers. Cancer Medicine publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.