Biomimetic chitosan-based scaffold for 3D breast cancer cell culture: a promising tool for anticancer drug screening

IF 3.4 4区工程技术 Q2 POLYMER SCIENCE

Macromolecular Research Pub Date : 2025-04-11 DOI:10.1007/s13233-025-00405-7

Agustina Setiawati, Nyoman Bayu Wisnu Kencana, Hendrik Satria Dwi Putra, Maria Violita Sekar Ayu Kencana, Olatunji Ajiteru, I Made Bayu Kresna Yoga, Nurul Fatimah, Adam Hermawan

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引用次数: 0

Abstract

Natural biodegradable polymers have been extensively studied as scaffolds for three-dimensional (3D) cancer cell culture in high-throughput screening (HTS) for anticancer drug discovery. This study fabricated a chitosan-based scaffold combined with pectin at different ratios: 10:90, 40:60, 60:40, and 90:10. Collagen I, the most abundant component of breast cancer extracellular matrix (ECM), was added to the scaffold formula. The composite scaffold displayed an interconnected, open-pore structure with tunable porosity, swelling, and degradable characteristics at different chitosan-to-pectin ratios. A high ratio of chitosan to pectin (60:40 and 90:10) exhibited the ideal properties for a 3D scaffold suitable for cell culture. These scaffolds supported the attachment and growth of the T47D breast cancer cell line. Additionally, this 3D cell culture demonstrated doxorubicin and tamoxifen resistance when compared to 2D culture. Therefore, it is a feasible and promising tool for more reliable anticancer drug screening.

Graphical abstract

Biomimetic scaffold supports 3D growth of breast cancer cells. This system mimics breast cancer tissue which grows on ECM networks, offering a personalized and better model for anticancer drug screening.

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基于仿生壳聚糖的3D乳腺癌细胞培养支架：一种有前途的抗癌药物筛选工具

天然生物可降解聚合物作为高通量筛选（HTS）抗癌药物发现的三维（3D）癌细胞培养支架已被广泛研究。本研究制备了不同比例的壳聚糖支架，分别为10:90、40:60、60:40和90:10。将乳腺癌细胞外基质（ECM）中最丰富的成分胶原I加入到支架配方中。复合支架具有相互连接的开孔结构，在不同壳聚糖与果胶的比例下具有可调节的孔隙率、溶胀率和可降解特性。壳聚糖与果胶的高比例（60:40和90:10）显示出适合细胞培养的3D支架的理想性能。这些支架支持T47D乳腺癌细胞系的附着和生长。此外，与2D培养相比，这种3D细胞培养表现出对阿霉素和他莫昔芬的耐药性。因此，它是一种可行的、有前景的、更可靠的抗癌药物筛选工具。仿生支架支持乳腺癌细胞三维生长。该系统模拟了在ECM网络上生长的乳腺癌组织，为抗癌药物筛选提供了个性化和更好的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Macromolecular Research 工程技术-高分子科学

CiteScore

4.70

自引率

8.30%

发文量

100

审稿时长

1.3 months

期刊介绍： Original research on all aspects of polymer science, engineering and technology, including nanotechnology Presents original research articles on all aspects of polymer science, engineering and technology Coverage extends to such topics as nanotechnology, biotechnology and information technology The English-language journal of the Polymer Society of Korea Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.