Polystyrene microplastic exposure alters the shell matrix proteome and compromises biomineralization in the freshwater snail Planorbarius corneus

IF 4.3 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-09-21 DOI:10.1016/j.aquatox.2025.107586

Chuang Liu, Guansong Chen, Jindong Xu

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Abstract

The continuous growth of plastic production makes microplastic pollution a globally significant environmental issue. Biomineralization, vital for mollusk growth and development, provides essential shell protection and support. However, studies on microplastic impacts on freshwater snail biomineralization remain scarce. This research exposed Planorbarius corneus snails to 5-μm polystyrene microplastics for 51 days. The control group exhibited significantly higher shell-width growth rates and egg-laying volumes than the exposed group. Proteomic analysis of shell matrix proteins (SMPs) via liquid chromatography-tandem mass spectrometry identified 213 SMPs in controls versus 189 in high-concentration exposed snails. Microplastics altered SMP composition and abundance, affecting tyrosinases, acidic matrix proteins, macroglobulins, and globins. This study reveals fundamental proteome-level disruptions in shell biomineralization caused by microplastics in freshwater snails.

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聚苯乙烯微塑料暴露改变了淡水蜗牛的壳基质蛋白质组和生物矿化

塑料生产的持续增长使微塑料污染成为一个全球性的重大环境问题。生物矿化对软体动物的生长和发育至关重要，提供了必要的外壳保护和支持。然而，关于微塑料对淡水蜗牛生物矿化影响的研究仍然很少。本研究将Planorbarius corneus蜗牛暴露在5 μm聚苯乙烯微塑料中51天。对照组的壳宽生长率和产蛋量显著高于暴露组。通过液相色谱-串联质谱法对壳基质蛋白（SMPs）进行蛋白质组学分析，在对照组中鉴定出213种SMPs，而在高浓度暴露的蜗牛中鉴定出189种SMPs。微塑料改变了SMP的组成和丰度，影响酪氨酸酶、酸性基质蛋白、巨球蛋白和珠蛋白。本研究揭示了淡水蜗牛中微塑料引起的外壳生物矿化的基本蛋白质组水平破坏。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.