{"title":"Localization of single molecules with structured illumination and structured detection.","authors":"Luciano A Masullo","doi":"10.1038/s41377-025-01980-1","DOIUrl":null,"url":null,"abstract":"A new high-precision single-molecule localization scheme, ISM-FLUX, is an implementation of MINFLUX using image-scanning microscopy (ISM) with a single-photon avalanche diode (SPAD) array detector. ISM-FLUX results in a larger localization range, enhancing the robustness of the localization scheme and it also potentially enables experiments in which absorption and emission of a single fluorophore can be probed independently.","PeriodicalId":18069,"journal":{"name":"Light-Science & Applications","volume":"27 1","pages":"347"},"PeriodicalIF":23.4000,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Light-Science & Applications","FirstCategoryId":"1089","ListUrlMain":"https://doi.org/10.1038/s41377-025-01980-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
A new high-precision single-molecule localization scheme, ISM-FLUX, is an implementation of MINFLUX using image-scanning microscopy (ISM) with a single-photon avalanche diode (SPAD) array detector. ISM-FLUX results in a larger localization range, enhancing the robustness of the localization scheme and it also potentially enables experiments in which absorption and emission of a single fluorophore can be probed independently.