Liquid chromatography-mass spectrometry-based metabolomics study of the protective mechanism of Shenji Guben decoction on chronic obstructive pulmonary disease.
Pan Tingyu, Yao Jing, G E Yue, Yang Shuang, Sun Zikai, Wei Yu, W U Jieyu, X U Yong, Zhou Xianmei, H E Hailang
{"title":"Liquid chromatography-mass spectrometry-based metabolomics study of the protective mechanism of Shenji Guben decoction on chronic obstructive pulmonary disease.","authors":"Pan Tingyu, Yao Jing, G E Yue, Yang Shuang, Sun Zikai, Wei Yu, W U Jieyu, X U Yong, Zhou Xianmei, H E Hailang","doi":"10.19852/j.cnki.jtcm.20250327.001","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanisms of the effect of Shenji Guben (SJGB) decoction on chronic obstructive pulmonary disease (COPD).</p><p><strong>Methods: </strong>A murine model of COPD was established through lipopolysaccharide (LPS) nasal drops and passive smoke exposure, followed by evaluation of SJGB decoction efficacy <i>via</i> lung function tests and histological analysis. Non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics was used to explore the mechanisms of SJGB decoction in COPD.</p><p><strong>Results: </strong>We found that the SJGB decoction effectively reduced inflammatory cell infiltration in the airways and lungs, and improved lung function in the COPD model mice. LC-MS-based metabolomics identified 86 biomarkers in COPD models. Compared to the model group, SJGB decoction significantly altered 34 metabolites. Prostaglandin E2 and DL-Citrulline were highlighted as two representative differential metabolites. MetaboAnalyst 5.0 highlighted glycerophospholipid and riboflavin metabolisms as key pathways affected by SJGB decoction.</p><p><strong>Conclusion: </strong>This study evaluated the protective effect of SJGB decoction against COPD and provided insights into its potential mechanisms in COPD treatment.</p>","PeriodicalId":94119,"journal":{"name":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","volume":"45 5","pages":"1009-1018"},"PeriodicalIF":0.0000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12453992/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.19852/j.cnki.jtcm.20250327.001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To investigate the mechanisms of the effect of Shenji Guben (SJGB) decoction on chronic obstructive pulmonary disease (COPD).
Methods: A murine model of COPD was established through lipopolysaccharide (LPS) nasal drops and passive smoke exposure, followed by evaluation of SJGB decoction efficacy via lung function tests and histological analysis. Non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics was used to explore the mechanisms of SJGB decoction in COPD.
Results: We found that the SJGB decoction effectively reduced inflammatory cell infiltration in the airways and lungs, and improved lung function in the COPD model mice. LC-MS-based metabolomics identified 86 biomarkers in COPD models. Compared to the model group, SJGB decoction significantly altered 34 metabolites. Prostaglandin E2 and DL-Citrulline were highlighted as two representative differential metabolites. MetaboAnalyst 5.0 highlighted glycerophospholipid and riboflavin metabolisms as key pathways affected by SJGB decoction.
Conclusion: This study evaluated the protective effect of SJGB decoction against COPD and provided insights into its potential mechanisms in COPD treatment.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
