Haptoglobin response in serum and pleural fluid of tuberculin reactor cattle assessed by culture and gross pathology

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal Pub Date : 2025-09-25 DOI:10.1016/j.tvjl.2025.106453

Anna Didkowska , Daniel Klich , Katarzyna Matusik , Magdalena Wilczek , Krzysztof Anusz , Monika Krajewska-Wędzina

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引用次数: 0

Abstract

Haptoglobin (Hp) serum concentration is a highly sensitive, non-specific indicator of inflammation. In the present study, following the example of studies conducted on humans, we analysed whether haptoglobin concentrations in pleural fluids and sera in tuberculin-reactor cattle could be an additional biomarker of Mycobacterium bovis infection. The clinical material (serum, n = 60, pleural fluid, n = 80) was collected from 140 dairy cattle (Bos taurus) shortly before culling at slaughter. During postmortem examination, organs were assessed for tuberculosis-like lesions and tissue samples were collected from all animals post-mortem for Mycobacterium tuberculosis complex (MTBC) culture. The relationship of Hp serum concentrations in cattle with and without tuberculous and the results of MTBC culture were analysed. Serum samples showed a positive correlation between the Hp concentrations and MTBC culture. However, this phenomenon was not confirmed in pleural fluids. Thus, Hp concentrations in cattle serum can be a useful complementary tuberculosis diagnostic tool in cattle.

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结核菌素反应牛血清和胸膜液中珠蛋白反应的培养和大体病理评价。

触珠蛋白（Hp）血清浓度是一种高度敏感、非特异性的炎症指标。在本研究中，根据对人类进行的研究，我们分析了结核菌反应牛胸膜液和血清中的接触珠蛋白浓度是否可能是牛分枝杆菌感染的另一个生物标志物。临床资料（血清，n=60，胸膜液，n=80）采集于140头奶牛（Bos taurus）屠宰前。在死后检查中，评估器官是否有结核样病变，并从所有死后动物身上收集组织样本进行结核分枝杆菌复合体（MTBC）培养。分析了有无结核牛血清Hp浓度与MTBC培养结果的关系。血清样品显示Hp浓度与MTBC培养呈正相关。然而，这一现象并未在胸膜液中得到证实。因此，牛血清中Hp浓度可作为牛结核病诊断的有用补充工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary journal 农林科学-兽医学

CiteScore

4.10

自引率

4.50%

发文量

审稿时长

40 days

期刊介绍： The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.