Xue Wang, Keerthi Booshan Manikandan, Hyug-Han Kim, Chang-Joon Kim
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引用次数: 0
Abstract
Glucose oxidase (GOx)-based electrodes offer promising applications in glucose sensing and as potential power sources for implantable devices, yet their performance remains critically dependent on efficient electron transfer and enzyme immobilization strategies. This study systematically investigated the co-immobilization of GOx and a redox-active osmium polymer, poly (N-vinylimidazole)-[Os(4,4′-dimethyl-2,2′-bipyridine)2Cl])+/2+ (PVI-Os-dme), using poly(ethylene glycol) diglycidyl ether (PEGDGE) as a crosslinker to enhance both the catalytic and electron-transfer properties of the electrode. By varying the enzyme-to-mediator ratio and applying a layer-by-layer assembly approach, we demonstrated that both loading quantity and composition critically influenced current generation, charge transfer resistance, and overall electrode efficiency. While current output increased with additional layers, the catalytic activity per unit mass of enzyme or mediator decreased, indicating a trade-off at high loadings. The optimized electrode, composed of six composite layers (2 μg GOx, 3.6 μg PVI-Os-dme, 2.2 μg PEGDGE per layer), achieved the highest peak current of 23.7 ± 1.7 μA at 0.3 V and retained over 85% of initial current after 3 cycles and 57% after 5 cycles, demonstrating favorable reusability. Kinetic analysis revealed an apparent Michaelis–Menten constant (Kmapp) of 9.0 mM and a maximum current (Imax) of 29.2 μA, confirming the electrode’s high affinity and catalytic efficiency toward glucose. These results highlight the importance of optimizing GOx/PVI-Os-dme loadings, ratio, and the number of layers for enhancing the electrode performance.
期刊介绍:
The Korean Journal of Chemical Engineering provides a global forum for the dissemination of research in chemical engineering. The Journal publishes significant research results obtained in the Asia-Pacific region, and simultaneously introduces recent technical progress made in other areas of the world to this region. Submitted research papers must be of potential industrial significance and specifically concerned with chemical engineering. The editors will give preference to papers having a clearly stated practical scope and applicability in the areas of chemical engineering, and to those where new theoretical concepts are supported by new experimental details. The Journal also regularly publishes featured reviews on emerging and industrially important subjects of chemical engineering as well as selected papers presented at international conferences on the subjects.