Development of a Safe and Effective mRNA Candidate Vaccine Against PEDV G2c Genotype Infection.

IF 3.5 3区医学 Q2 VIROLOGY

Viruses-Basel Pub Date : 2025-09-04 DOI:10.3390/v17091210

Shixuan Zhu, Nan Cao, Huawei Zhang, Leqiang Sun

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引用次数: 0

Abstract

Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe diarrhea, dehydration, and high mortality in piglets, leading to significant economic losses in the swine industry. The spike (S) protein of PEDV is the primary target for neutralizing antibodies and is critical for vaccine development. In this study, the pUC57-S01 and pUC57-S02 plasmids carrying the codon-optimized truncated S gene sequence were constructed. The mRNA S01 showed higher protein expression in vitro than mRNA S02, as confirmed by Western blotting. The safety and immunogenicity of mRNA S01 were evaluated in animal experiments. The results indicated that the mRNA S01 vaccine was safe for piglets and pregnant sows. Immunogenicity was assessed by a neutralization assay, which revealed that encapsulated mRNA S01 induced high levels of neutralizing antibody titers in pigs. Challenge protection efficiency tests showed that the mRNA S01 vaccine conferred immunity to newborn piglets, protecting them from a homologous PEDV strain challenge. This study provides a foundation for the clinical application of PEDV mRNA vaccines and offers a reference for the development of novel vaccines against PEDV.

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一种安全有效的抗PEDV G2c基因型感染mRNA候选疫苗的研制

猪流行性腹泻病毒（PEDV）是一种高度传染性的冠状病毒，可导致仔猪严重腹泻、脱水和高死亡率，给养猪业造成重大经济损失。PEDV的刺突(S)蛋白是中和抗体的主要靶点，对疫苗的开发至关重要。本研究构建了携带密码子优化截断S基因序列的pUC57-S01和pUC57-S02质粒。Western blotting证实，mRNA S01在体外的蛋白表达高于mRNA S02。动物实验对mRNA S01的安全性和免疫原性进行了评价。结果表明，mRNA S01疫苗对仔猪和妊娠母猪是安全的。免疫原性通过中和试验评估，结果表明，包封的mRNA S01在猪体内诱导了高水平的中和抗体滴度。攻击保护效果试验表明，mRNA S01疫苗对新生仔猪具有免疫作用，保护仔猪免受同源PEDV毒株的攻击。本研究为PEDV mRNA疫苗的临床应用奠定了基础，并为PEDV新型疫苗的开发提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.