Effects of Dietary Bacillus subtilis and Bacteriophage Supplementation on Water Quality, Carcass Traits, and Muscle Growth in Magang Geese.

IF 2.3 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary Sciences Pub Date : 2025-09-04 DOI:10.3390/vetsci12090861

Yong Li, Yongquan Luo, Yuanhao Han, Zhiyuan Liu, Songchao Li, Xiujin Li, Zhongping Wu, Yunbo Tian, Yunmao Huang, Xumeng Zhang

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引用次数: 0

Abstract

Background: Escherichia coli and Salmonella contamination in goose bath water releases endotoxins like lipopolysaccharide (LPS), compromising immunity and hindering goose farming.

Objective: This study evaluated effects of dietary Bacillus subtilis and bacteriophage supplementation on water quality, carcass traits, and muscle growth in Magang geese.

Method: A total of 288 geese were divided into four groups based on similarity in weight (n = 6 geese): A (basal diet); B (basal diet + bacteriophage: 5.0 × 10¹⁰ PFU/L at 1:1000 dilution); C (basal diet + Bacillus subtilis: 5.0 × 10⁹ CFU/kg); D (basal diet + bacteriophage + Bacillus subtilis).

Results: Supplementation significantly increased wing length, tibia length, and live weight at 60 days. It reduced water and plasma endotoxin levels and suppressed viable counts of Escherichia coli, Salmonella, and total bacteria in water across rearing stages. Supplementation up-regulated mRNA and protein expression of myogenic regulators (MYOD, MYOG, MYH1) and IGF-1, while down-regulating pro-inflammatory cytokines (TNF-α, IL-6), suggesting enhanced myofiber growth.

Conclusion: These findings demonstrate that Bacillus subtilis and bacteriophage supplementation improves goose growth performance and immune status by modulating key genes, reducing pathogens and endotoxins, offering an eco-friendly strategy to enhance productivity and potentially reduce antibiotic dependency.

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饲粮中添加枯草芽孢杆菌和噬菌体对马钢鹅水质、胴体性状和肌肉生长的影响。

背景：鹅浴水中的大肠杆菌和沙门氏菌污染会释放内毒素，如脂多糖（LPS），损害免疫力，阻碍鹅养殖。目的：研究饲粮中添加枯草芽孢杆菌和噬菌体对马钢鹅水质、胴体性状和肌肉生长的影响。方法：288只鹅按体重相似度分为4组（n = 6只）：A组（基础饲粮）；B（基础日粮+噬菌体：5.0 × 1010 PFU/L， 1:1000稀释）；C（基础饲粮+枯草芽孢杆菌：5.0 × 109 CFU/kg）；D（基础日粮+噬菌体+枯草芽孢杆菌）。结果：添加物显著增加了60日龄鸡的翼长、胫骨长和活重。它降低了水和血浆内毒素水平，并抑制了大肠杆菌、沙门氏菌和水中总细菌的活菌数。肌生成调节因子（MYOD、MYOG、MYH1）和IGF-1 mRNA和蛋白表达上调，促炎因子（TNF-α、IL-6）表达下调，提示肌纤维生长增强。结论：这些研究结果表明，添加枯草芽孢杆菌和噬菌体通过调节关键基因、减少病原体和内毒素来改善鹅的生长性能和免疫状态，为提高产量和减少抗生素依赖提供了一种生态策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.