Evaluation of the Preoperative Antiseptic Efficacy of Ozone on Dog Skin in Comparison with Traditional Methods.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Pierre Melanie, Carlotta Niola, Federico Guerrini, Nicolò Pareto, Simone Mancini, Filippo Fratini
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引用次数: 0

Abstract

This paper aims to assess the antiseptic capacity of a double-distilled ozonated water solution and compare it to standard presurgical antiseptic protocols based on chlorhexidine and alcohol. Sixty-three canine patients undergoing different surgical procedures (such as thoracic, abdominal, proximal limb, or cervical) were enrolled in the study. Following extensive preoperative clipping of the selected anatomical regions, four standardized skin areas (6 × 6 cm each) were aseptically sampled according to a predefined protocol: Area 1 received soap-based chlorhexidine followed by alcoholic chlorhexidine; Area 2 was prepared using a combination of soap-based chlorhexidine and ozonated water; Areas 3 and 4 underwent presurgical skin antisepsis with a combination of ethyl alcohol and ozonated water, and ozonated water alone, respectively. Two different samples per area were collected using bacteriological buffers in order to evaluate the bacterial count at 90 s and 180 s, respectively. Those samples were sown via streak plating on Petri dishes using PCA (plate count agar) as the medium. The determination of the total mesophilic bacterial count on Petri dishes was used to assess the efficacy of the antiseptics in different areas. Results show that ozonated bidistilled water demonstrated comparable or superior efficacy compared to other antiseptics tested in this study. Its integration into surgical protocols could enhance patient safety while controlling environmental concerns associated with the massive use of chemical antiseptics.

术前臭氧对犬皮肤消毒效果与传统方法的比较。
本文旨在评估双蒸馏臭氧化水溶液的灭菌能力,并将其与基于氯己定和酒精的标准手术前灭菌方案进行比较。63名犬患者接受了不同的外科手术(如胸部、腹部、近端肢体或颈部),参与了这项研究。在术前对选定的解剖区域进行广泛的剪裁后,根据预先确定的方案,对四个标准化的皮肤区域(每个区域6 × 6 cm)进行无菌取样:区域1接受肥皂基氯己定,然后接受酒精氯己定;区域2采用皂基氯己定与臭氧化水混合制备;手术前,3区和4区分别使用乙醇和臭氧水和单独使用臭氧水进行皮肤消毒。每个区域用细菌缓冲液收集两个不同的样品,分别评估90 s和180 s时的细菌计数。这些样品以PCA(平板计数琼脂)为培养基,通过条纹镀在培养皿上播种。通过对培养皿中嗜温细菌总数的测定,对不同地区的抗菌剂效果进行评价。结果表明,与本研究中测试的其他防腐剂相比,臭氧化双蒸馏水显示出相当或更好的功效。将其整合到手术方案中可以提高患者安全,同时控制与大量使用化学防腐剂相关的环境问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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