Leaf Nitrogen Allocation Trade-Offs Promote Efficient Utilization of Different Nitrogen Forms in Hemarthria altissima.

Abstract

The sharp increase in atmospheric nitrogen deposition has had profound effects on nitrogen availability and the photosynthetic capacity of terrestrial plants. Consequently, understanding the intricate trade-off between nitrogen sources and their allocation within leaves is essential for unraveling the photosynthetic responses of grassland ecosystems to nitrogen deposition. In a series of field experiments, the effects of different nitrogen forms (ammonium and nitrate nitrogen) on nitrogen assimilation and allocation in the C₄ plant Hemarthria altissima were thoroughly investigated. Towards the end of the growing season, H. altissima was observed to exhibit high photosynthetic efficiency. Ammonium nitrogen treatment notably enhanced photosynthetic nitrogen use efficiency (PNUE) by modifying the nitrogen allocation within the leaf's photosynthetic apparatus and leaf area, leading to a significant improvement in photosynthetic efficiency and biomass accumulation. Under ammonium nitrogen treatment, H. altissima directed more nitrogen toward its carboxylation process and other protein-related functions to increase carboxylation efficiency, thereby facilitating the accumulation of photosynthetic products. In contrast, under nitrate nitrogen treatment, the plant balanced growth and light absorption by allocating nitrogen to leaf light-capturing proteins. The application of both ammonium and nitrate nitrogen resulted in increased nitrogen content in the soil, as ammonium nitrogen is converted to nitrate nitrogen through nitrification. The net photosynthetic rate (A_n), nitrogen allocation to photosynthetic components (N_psn), and chlorophyll content per unit area (Chl_area) were all significantly and positively correlated with photosynthetic nitrogen use efficiency (PNUE). Notably, under the sole NH₄⁺ treatment, nitrogen allocation to the photosynthetic components increased, which enhanced the NPQ and ETR in H. altissima leaves. These findings suggest that H. altissima preferentially utilizes ammonium nitrogen from the soil, optimizing its PNUE and biomass accumulation through a strategic allocation of nitrogen within its leaves. Further investigation is needed to explore how these nitrogen allocation strategies may vary under different environmental conditions and how they influence ecosystem-level productivity.