Visual Detection of Malaria Parasite-Parasitized Erythroblasts in Peripheral Blood via Immunization-Based Model.

IF 5.2 3区医学 Q1 IMMUNOLOGY

Vaccines Pub Date : 2025-09-21 DOI:10.3390/vaccines13090988

Kumpei Ito, Yuki S Tateishi, Takashi Imai, Shinya Miyazaki, Yukiko Miyazaki, Wataru Kagaya, Mai Nakashima, Miho Sase, Misato Yoshioka-Takeda, Chikako Shimokawa, Kyoko Hayashi, Kentaro Itokawa, Osamu Komagata, Ha Ngo-Thanh, Aoi Shimo, Tamasa Araki, Takeshi Annoura, Takashi Murakami, Hajime Hisaeda

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引用次数: 0

Abstract

Background: Erythroblasts have recently been identified as host cells for malarial parasites, revealing a previously underappreciated host-parasite interaction. However, their extremely low abundance in peripheral blood has hindered progress, especially in elucidating the biological significance of parasitized erythroblasts (pEBs) in vivo.

Methods: Here, we visualized pEBs in a murine model and established a method to increase their number in peripheral blood by immunizing mice with live Plasmodium yoelii 17XNL, followed by challenge with P. berghei ANKA.

Results: Immunized mice were protected from cerebral malaria and survived longer, during which pEBs appeared in circulation and were detected using Giemsa-stained smears. All blood-stage parasite forms were identified within pEBs, including enucleating erythroblasts.

Conclusions: This model enables in vivo/ex vivo analysis of pEB biology without bone marrow/spleen isolation, thus lowering technical/ethical barriers for the field.

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基于免疫模型的外周血疟疾寄生虫寄生红细胞视觉检测。

背景：红细胞最近被鉴定为疟原虫的宿主细胞，揭示了以前未被重视的宿主-寄生虫相互作用。然而，它们在外周血中的极低丰度阻碍了进展，特别是在阐明寄生红细胞（pEBs）在体内的生物学意义方面。方法：我们在小鼠模型中可视化peb，并建立了一种方法，即用活的约氏疟原虫17XNL免疫小鼠，然后用伯氏疟原虫ANKA攻毒，从而增加peb在外周血中的数量。结果：免疫小鼠对脑疟疾具有保护作用，存活时间更长，在此期间，peb出现在循环中，并通过吉姆萨染色涂片检测到。所有血期寄生虫形式在peb中被鉴定，包括去核的红母细胞。结论：该模型可以在不分离骨髓/脾脏的情况下进行pEB生物学的体内/体外分析，从而降低了该领域的技术/伦理障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vaccines Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

8.90

自引率

16.70%

发文量

1853

审稿时长

18.06 days

期刊介绍： Vaccines (ISSN 2076-393X) is an international, peer-reviewed open access journal focused on laboratory and clinical vaccine research, utilization and immunization. Vaccines publishes high quality reviews, regular research papers, communications and case reports.