Laponite®-Based Smart Hydrogels for Sustained Topical Delivery of Silver Sulfadiazine: A Strategy for the Treatment of Contaminated or Biofilm-Forming Wounds.

IF 5.5 3区 医学 Q1 PHARMACOLOGY & PHARMACY
Jonas Lira do Nascimento, Michely Conceição Viana da Costa, Leticia Farias de Macêdo, Luiz Henrique Chaves de Macêdo, Ricardo Olímpio de Moura, Tomás Jeferson Alves de Mélo, Wilma Raianny Vieira da Rocha, Ana Cristina Figueiredo de Melo Costa, José Lamartine Soares-Sobrinho, Dayanne Tomaz Casimiro da Silva
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引用次数: 0

Abstract

Background/Objectives: Silver sulfadiazine (AgSD) is widely used in the topical treatment of burns and infected wounds, but its conventional formulations present drawbacks such as poor water solubility, the need for multiple daily applications, and patient discomfort. To overcome these limitations, this study aimed to develop and evaluate Laponite® (LAP)-based hydrogels loaded with AgSD for controlled release and enhanced antimicrobial and antibiofilm efficacy, offering a promising alternative for the treatment of contaminated or biofilm-forming wounds. Methods: Laponite®-based hydrogels containing 1% and 1.2% AgSD (LAP@AgSD) were prepared using a one-pot method. The formulations were characterized rheologically, thermally, and structurally. In vitro drug release was assessed using Franz diffusion cells, and mathematical modeling was applied to determine release kinetics. Antibacterial and antibiofilm activities were evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using standardized microbiological methods. Results: LAP@AgSD hydrogels exhibited pseudoplastic behavior, high structural integrity, and enhanced thermal stability. In vitro release assays revealed a sustained release profile, best fitted by the Weibull model, indicating diffusion-controlled mechanisms. Antibacterial assays demonstrated concentration-dependent activity, with LAP@AgSD 1.2% showing superior efficacy over LAP@AgSD 1% and comparable performance to the commercial silver sulfadiazine cream (CC-AgSD). Biofilm inhibition was significant for all formulations, with CC-AgSD 1% exhibiting the highest immediate activity, while LAP@AgSD 1.2% provided sustained antibiofilm potential. Conclusions: LAP-based hydrogels are promising smart delivery systems for AgSD, combining mechanical robustness, controlled drug release, and effective antibacterial and antibiofilm activities. These findings support their potential use in topical therapies for infected and chronic wounds, particularly where biofilm formation is a challenge.

基于Laponite®的智能水凝胶,用于持续局部递送磺胺嘧啶银:一种治疗污染或生物膜形成伤口的策略。
背景/目的:磺胺嘧啶银(AgSD)广泛用于烧伤和感染创面的局部治疗,但其传统配方存在水溶性差、需要每日多次使用以及患者不适等缺点。为了克服这些限制,本研究旨在开发和评估负载AgSD的Laponite®(LAP)基水凝胶,用于控释和增强抗菌和抗生物膜功效,为治疗污染或生物膜形成的伤口提供了一种有希望的替代方案。方法:采用一锅法制备含1%和1.2% AgSD的Laponite®基水凝胶(LAP@AgSD)。对该配方进行了流变性、热学和结构表征。采用Franz扩散细胞法测定体外药物释放,并建立数学模型确定释放动力学。采用标准化的微生物学方法对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌和抗生物膜活性进行了评价。结果:LAP@AgSD水凝胶表现出假塑性行为、高结构完整性和增强的热稳定性。体外释放试验显示缓释谱,最适合Weibull模型,表明扩散控制机制。抗菌试验显示出浓度依赖性活性,LAP@AgSD 1.2%的抗菌效果优于LAP@AgSD 1%,与商业磺胺嘧啶银乳膏(CC-AgSD)的性能相当。所有配方的生物膜抑制作用都很显著,其中1%的CC-AgSD表现出最高的直接活性,而LAP@AgSD 1.2%则提供了持续的抗生物膜潜力。结论:基于lap的水凝胶具有机械稳健性、药物释放可控、有效的抗菌和抗生物膜活性等优点,是一种很有前景的AgSD智能给药系统。这些发现支持了它们在感染和慢性伤口的局部治疗中的潜在应用,特别是在生物膜形成是一个挑战的地方。
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来源期刊
Pharmaceutics
Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍: Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications,  and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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