Therapeutic Approach Based on Nanotechnology with Chitosan-Coated Zein Nanoparticles Containing Quercetin Against Resistant Klebsiella pneumoniae Clinical Isolates.

IF 5.5 3区 医学 Q1 PHARMACOLOGY & PHARMACY
Azael Francisco Silva-Neto, Maria Anndressa Alves Agreles, Ana Alice Venancio Correia, Hanne Lazla Rafael de Queiroz Macêdo, Alane Rafaela de Carvalho Amaral, Alexsandra Maria Lima Scavuzzi, João Victor de Oliveira Alves, Ana Catarina Souza Lopes, Márcia Vanusa da Silva, Maria Tereza Dos Santos Correia, Isabella Macário Ferro Cavalcanti, Luís André de Almeida Campos
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引用次数: 0

Abstract

Background/Objectives: The study developed, characterized, and evaluated the toxicity, antibacterial and antibiofilm activity of quercetin encapsulated in chitosan-coated zein nanoparticles (QUER-ZNP-CH). Methods: QUER-ZNP-CH were prepared by the nanoprecipitation method and characterized by physicochemical analyses, stability (12 months), and release kinetics. Toxicity was evaluated through hemocompatibility and a Tenebrio molitor larval model. Antibacterial activity (MIC/MBC, CLSI) and antibiofilm potential (crystal violet assay) were tested against resistant Klebsiella pneumoniae strains. Results: The nanoparticles were prepared, and physicochemical analyses revealed chemical interactions, efficient encapsulation of the drug, and thermal stability. The formulations remained stable over 12 months, and the release kinetics demonstrated controlled release for 72 h. No hemotoxic profile was observed and there was 95% survival of Tenebrio molitor larvae after treatment with QUER-ZNP-CH. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of QUER-ZNP-CH revealed enhanced antibacterial activity of QUER, as indicated by a 32 to 64-fold reduction in the MIC and MBC values. The biofilm inhibition potential of QUER-ZNP-CH showed 60-100% inhibition and 25-95% eradication in concentrations from 0.12 to 62.5 μg/mL. Conclusions: Thus, this nanotechnology-based formulation suggests potential for the treatment of bacterial infections caused by multidrug-resistant K. pneumoniae strains.

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壳聚糖包被含有槲皮素的玉米蛋白纳米颗粒纳米技术治疗耐药肺炎克雷伯菌临床分离株
背景/目的:研究壳聚糖包被玉米蛋白纳米颗粒(QUER-ZNP-CH)中槲皮素的毒性、抗菌和抗生物膜活性,并对其进行表征和评价。方法:采用纳米沉淀法制备QUER-ZNP-CH,并对其进行理化分析、稳定性(12个月)和释放动力学表征。通过血液相容性和黄粉虫幼虫模型评价毒性。对耐药肺炎克雷伯菌进行抑菌活性(MIC/MBC、CLSI)和抗菌膜电位(结晶紫法)测定。结果:制备出纳米颗粒,理化分析表明纳米颗粒具有化学相互作用、包封效果好、热稳定性好等特点。配方在12个月内保持稳定,释放动力学显示72 h控释。未观察到血液毒性,QUER-ZNP-CH处理后的黄粉虫幼虫存活率为95%。最小抑菌浓度(MIC)和最小杀菌浓度(MBC)表明,QUER的抑菌活性增强,MIC和MBC值降低32 ~ 64倍。在0.12 ~ 62.5 μg/mL浓度范围内,QUER-ZNP-CH的生物膜抑制电位为60-100%,根除率为25-95%。结论:因此,这种基于纳米技术的制剂可能用于治疗多重耐药肺炎克雷伯菌菌株引起的细菌感染。
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来源期刊
Pharmaceutics
Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍: Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications,  and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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