Effect of 6-Shogaol Derived from Ginger (Zingiber officinale) on Dual-Species Biofilm Formation by Streptococcus mutans and Candida albicans.

IF 5 2区 医学 Q1 NUTRITION & DIETETICS
Nutrients Pub Date : 2025-09-19 DOI:10.3390/nu17182999
Eun-Ha Jung, Geelsu Hwang, Ki-Rim Kim
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引用次数: 0

Abstract

Background/objectives: Dental plaque, a biofilm composed of accumulated oral microorganisms, is a key contributor to various oral diseases. 6-shogaol, a bioactive compound of ginger, is known to have pharmacological activities, including anticancer, anti-inflammatory, and antimicrobial activities. Therefore, we aimed to determine the effects of 6-shogaol on dual-species biofilms of Streptococcus mutans (S. mutans) and Candida albicans (C. albicans).

Methods: Dual-species oral biofilms were formed on hydroxyapatite (HA) disks for 42 h and exposed to 6-shogaol. The pH was measured in the experimental medium, and the biomass, colony-forming unit (CFU) of microbial cells, and insoluble extracellular polysaccharides (EPS) were quantified in the biofilm formed on the HA disk. Confocal laser scanning microscopy (CLSM) was used to assess biofilm morphology, and quantitative polymerase chain reaction was performed to analyze gtf gene expression.

Results: 6-shogaol dose-dependently reduced insoluble EPS, CFU counts, and dry weight of biofilms. The pH was maintained above 5.5 in the 6-shogaol-treated group. CLSM images showed that S. mutans proliferation, C. albicans hyphal development, and EPS production were markedly inhibited in biofilms treated with 6-shogaol. The expression of gtfB and gtfC was significantly downregulated by 6-shogaol.

Conclusions: These findings suggest that 6-shogaol has the potential to be a promising natural product for the prevention and management of oral biofilm-related oral diseases.

生姜提取物6-Shogaol对变形链球菌和白色念珠菌双种生物膜形成的影响
背景/目的:牙菌斑是口腔微生物聚集形成的生物膜,是导致多种口腔疾病的重要因素。6-姜酚是生姜的一种生物活性化合物,具有抗癌、抗炎和抗菌等药理活性。因此,我们旨在确定6-shogaol对变形链球菌(S. mutans)和白色念珠菌(C. albicans)双种生物膜的影响。方法:双物种口腔生物膜在羟基磷灰石(HA)圆盘上形成42 h,暴露于6-shogaol。测定实验培养基的pH值,并定量测定HA圆盘上形成的生物膜中微生物细胞的生物量、菌落形成单位(CFU)和不溶性细胞外多糖(EPS)。用共聚焦激光扫描显微镜(CLSM)观察生物膜形态,用定量聚合酶链反应分析gtf基因表达。结果:6-shogaol剂量依赖性地减少了生物膜的不溶性EPS、CFU计数和干重。6-shogaol处理组pH维持在5.5以上。CLSM图像显示,6-shogaol处理的生物膜明显抑制了变形链球菌的增殖、白色念珠菌的菌丝发育和EPS的产生。6-shogaol显著下调gtfB和gtfC的表达。结论:6-shogaol有潜力成为一种预防和治疗口腔生物膜相关口腔疾病的天然产物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nutrients
Nutrients NUTRITION & DIETETICS-
CiteScore
9.20
自引率
15.30%
发文量
4599
审稿时长
16.74 days
期刊介绍: Nutrients (ISSN 2072-6643) is an international, peer-reviewed open access advanced forum for studies related to Human Nutrition. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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