Dose-Dependent Effect of Tilmicosin Residues on ermA Rebound Mediated by IntI1 in Pig Manure Compost.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2025-09-11 DOI:10.3390/microorganisms13092123

Pengfei Zhang, Qingnan Mo, Chang Liu, Qing Liu, Jiaojiao Xu, Yan Wang, Xin Wen, Yinbao Wu

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引用次数: 0

Abstract

The impact of varying antibiotic residue levels on antibiotic resistance gene (ARG) removal during composting is still unclear. This study investigated the impact of different residue levels of tilmicosin (TIM), a common veterinary macrolide antibiotic, on ARG removal during pig manure composting. Three groups were used: the CK group (no TIM), the L group (246.49 ± 22.83 mg/kg TIM), and the H group (529.99 ± 16.15 mg/kg TIM). Composting removed most targeted macrolide resistance genes (MRGs) like ereA, ermC, and ermF (>90% removal), and reduced ermB, ermX, ermQ, acrA, acrB, and mefA (30-70% removal). However, ermA increased in abundance. TIM altered compost community structure, driving succession through a deterministic process. At low doses, TIM reduced MRG-bacteria co-occurrence, with horizontal gene transfer via intI1 being the main cause of ermA rebound. In conclusion, composting reduces many MRG levels in pig manure, but the persistence and rebound of genes like ermA reveal the complex interactions between composting conditions and microbial gene transfer.

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替尔米科星残留对猪粪堆肥中IntI1介导的ermA反弹的剂量依赖性影响

不同的抗生素残留水平对堆肥过程中抗生素抗性基因（ARG）去除的影响尚不清楚。本研究探讨了猪粪便堆肥过程中，不同残留量的兽用大环内酯类抗生素替米霉素（TIM）对ARG去除的影响。试验分为3组：CK组（无TIM）、L组（246.49±22.83 mg/kg TIM）和H组（529.99±16.15 mg/kg TIM）。堆肥去除了大部分靶向大环内酯类耐药基因（mrg），如ereA、ermC和ermF（去除率为90%），并降低了ermB、ermX、ermQ、acrA、acrB和mefA（去除率为30-70%）。然而，ermA丰度增加。TIM改变了堆肥群落结构，通过确定性过程驱动演替。在低剂量下，TIM减少了mrg -细菌的共发生，通过intI1的水平基因转移是ermA反弹的主要原因。综上所述，堆肥降低了猪粪中许多MRG水平，但ermA等基因的持续和反弹揭示了堆肥条件与微生物基因转移之间的复杂相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.