Development of a Chemiluminescence Immunoassay for the Serological Diagnosis of Sheep and Bovine Brucellosis.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2025-09-22 DOI:10.3390/microorganisms13092214

Xin Yan, Mingze Chen, Yuning Liu, Mingjun Sun, Mengkun Huang, Jihui Jin, Jiaqi Li, Xiangxiang Sun, Mengda Liu, Haobo Zhang, Weixing Shao, Shufang Sun, Xiaoxu Fan, Wenlong Nan

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Abstract

Brucellosis, a zoonotic infection caused by the intracellular pathogen Brucella, leads to chronic multi-organ damage. Currently, rapid, accurate, and sensitive diagnostic technologies are crucial for the prevention and control of brucellosis. This study describes the development of a chemiluminescent immunoassay (Bru-CLIA) for sheep and bovine brucellosis antibody detection, utilizing Brucella abortus strain A19 lipopolysaccharide-coated magnetic particles (LPS-MPs) as the serum antigen and acridinium ester-labeled recombinant streptococcal protein G (AE-SPG) for signal generation. After optimizing the assay's parameters, the Bru-CLIA demonstrated a sensitivity of approximately 1 IU/mL and 2 IU/mL for detecting sheep and bovine brucellosis, respectively. No cross-reactivity was observed with sera from animals immunized with Escherichia coli O157:H7, Mycobacterium tuberculosis, Vibrio cholerae, Legionella, Salmonella, Foot and Mouth Disease virus types O and A, Bovine viral diarrhea virus, Sheep contagious pleuropneumonia, Goat pox virus, or Peste des Petits Ruminants virus, indicating strong specificity. The testing of 81 sheep serum samples and 96 bovine serum samples revealed that Bru-CLIA showed 87.65% and 93.75% concordance with the ID-VET commercial kits for sheep and bovine brucellosis detection, respectively. These results demonstrate that Bru-CLIA offers high specificity, sensitivity, repeatability, and reliability, making it a viable rapid diagnostic tool for the epidemiological surveillance of brucellosis.

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绵羊和牛布鲁氏菌病血清学诊断化学发光免疫分析法的建立。

布鲁氏菌病是一种由细胞内病原体布鲁氏菌引起的人畜共患感染，可导致慢性多器官损伤。目前，快速、准确和敏感的诊断技术对于预防和控制布鲁氏菌病至关重要。本研究利用流产布鲁氏菌菌株A19脂多糖包被磁颗粒（LPS-MPs）作为血清抗原，用acridiium酯标记重组链球菌蛋白G （AE-SPG）作为信号产生，建立了羊和牛布鲁氏菌抗体检测的化学发光免疫分析法（brul - clia）。优化检测参数后，布鲁氏菌法检测绵羊和牛布鲁氏菌病的灵敏度分别约为1 IU/mL和2 IU/mL。与O157:H7大肠杆菌、结核分枝杆菌、霍乱弧菌、军团菌、沙门氏菌、O型和A型口蹄疫病毒、牛病毒性腹泻病毒、绵羊传染性胸膜肺炎、山羊痘病毒、小反刍兽疫病毒免疫动物血清无交叉反应，特异性强。81份羊血清和96份牛血清与ID-VET羊、牛布鲁氏菌病商品化检测试剂盒的一致性分别为87.65%和93.75%。结果表明，Bru-CLIA具有较高的特异性、敏感性、重复性和可靠性，是一种可行的布鲁氏菌病流行病学监测快速诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.