Non-Invasive Three-Dimensional Cell Manipulation Technology Based on Acoustic Microfluidic Chips.

IF 3 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Micromachines Pub Date : 2025-09-22 DOI:10.3390/mi16091068

Lin Lin, Yiming Zhen, Wang Li, Guoqiang Dong, Rongxing Zhu, Minhui Liang

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引用次数: 0

Abstract

This study presents a non-invasive three-dimensional cell manipulation technique based on acoustic microfluidic chips, which generates acoustic flow fields through the vibration of micropillars induced by bulk acoustic waves to achieve precise multi-dimensional rotational manipulation of cells. Moreover, the characteristics of the acoustic flow field under linear, quasi-circular, elliptical, and higher-order vibration modes were intensively studied, and the rotational manipulation performance of polystyrene microbeads and cancer cells was optimized by adjusting the frequency and voltage. The results showed that the rotational speed and direction of the particles varied significantly in different vibration modes, with the particles and cells achieving the highest rotational speed in the elliptical vibration mode (frequency: 44.9 kHz, and voltage: 60 Vpp). In addition, the technique successfully achieved in-plane and out-of-plane rotation of cancer cells, and cell viability tests showed that 94% of the cells remained active after manipulation, demonstrating the low damage and biocompatibility of the method. This study provides a new, efficient, precise and gentle approach to three-dimensional manipulation of cells, which holds significant potential in biomedical research and clinical applications.

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基于声学微流控芯片的无创三维细胞操作技术。

本研究提出了一种基于声学微流控芯片的无创三维细胞操纵技术，该技术通过体声波诱导微柱振动产生声流场，实现对细胞的高精度多维旋转操纵。此外，深入研究了线性、准圆形、椭圆和高阶振动模式下的声流场特性，并通过调节频率和电压优化了聚苯乙烯微珠和癌细胞的旋转操纵性能。结果表明：在不同的振动模式下，颗粒的旋转速度和方向变化显著，在椭圆振动模式下（频率为44.9 kHz，电压为60 Vpp），颗粒和细胞的旋转速度最高；此外，该技术成功实现了癌细胞的平面内和平面外旋转，细胞活力测试显示，94%的细胞在操作后仍保持活性，证明了该方法的低损伤和生物相容性。本研究提供了一种新的、高效的、精确的、温和的细胞三维操作方法，在生物医学研究和临床应用中具有重要的潜力。

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来源期刊

Micromachines NANOSCIENCE & NANOTECHNOLOGY-INSTRUMENTS & INSTRUMENTATION

CiteScore

5.20

自引率

14.70%

发文量

1862

审稿时长

16.31 days

期刊介绍： Micromachines (ISSN 2072-666X) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to micro-scaled machines and micromachinery. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.