Microbial-Modified Organosilica-Based Dual-Mode Fluorescent Probe for Zn²⁺ and pH Sensing with Potential Application in Gut Immunity Modulation.

IF 3.1 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2025-09-27 DOI:10.1007/s10895-025-04573-8

Qing-Qing Han, Fang Chen, Zhao-Lin Zhang, Dong-Ya Chen

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引用次数: 0

Abstract

Crohn's disease (CD) involves chronic intestinal inflammation, often accompanied by abnormal zinc ion (Zn²⁺) homeostasis. Herein, we developed a multifunctional nanoplatform-1-DASA-ATPMS@2@Rg3-that integrates ginsenoside Rg3 with a DASA-modified silica-based composite for ratiometric Zn²⁺ sensing and anti-inflammatory therapy. The nanocomposite exhibited a distinct ratiometric fluorescence response to Zn²⁺, with a strong linear correlation (R² = 0.9969) between the intensity ratio (I₅₀₀/I₆₇₂) and Zn²⁺ concentration over 0-200 µM, enabling accurate quantification. Time-resolved fluorescence and zeta potential analyses confirmed Zn²⁺-induced coordination and electrostatic interactions, contributing to signal amplification. Additionally, the pH-dependent optical properties further support its adaptability to inflamed gut environments. This dual-functional nanoplatform not only enables precise Zn²⁺ monitoring-a crucial marker in CD progression-but also offers therapeutic benefits via Rg3-mediated immunomodulation, presenting a promising strategy for real-time inflammation sensing and intervention in CD.

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微生物修饰的有机硅基双模荧光探针用于Zn 2 +和pH传感在肠道免疫调节中的潜在应用。

克罗恩病（CD）涉及慢性肠道炎症，通常伴有锌离子（Zn 2 +）体内平衡异常。在这里，我们开发了一种多功能nanoplatform-1-DASA-ATPMS@2@Rg3，它将人参皂苷Rg3与dasa修饰的硅基复合材料结合在一起，用于比例Zn 2 +传感和抗炎治疗。纳米复合材料对Zn 2 +表现出明显的比例荧光响应，强度比（I₅₀₀/I₆₇₂）与Zn 2 +的浓度在0-200µM以上具有很强的线性相关性（R²= 0.9969），可以精确定量。时间分辨荧光和zeta电位分析证实了Zn 2 +诱导的配位和静电相互作用，有助于信号放大。此外，ph依赖的光学特性进一步支持其对炎症肠道环境的适应性。这种双功能纳米平台不仅可以实现精确的Zn 2 +监测（CD进展的关键标志物），还可以通过rg3介导的免疫调节提供治疗效果，为CD的实时炎症感知和干预提供了一种有希望的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.