Optimization of Manuka Honey microbubble particle structures for enhanced inhibition of Pseudomonas aeruginosa growth

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-24 DOI:10.1016/j.mimet.2025.107278

Pei-Ju Lin

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引用次数: 0

Abstract

This study aims to optimize the use of Manuka Honey (MH) microbubbles for enhancing antimicrobial efficacy in the treatment of Pseudomonas aeruginosa (P. aeruginosa.) infections. The experimental design included three different MH microbubble particle sizes produced by three types of high-density stainless steel mesh layers, two types of dressing, two volumes of use, and three antimicrobial exposure times. The bacterial survival percentages of P. aeruginosa survival obtained under each combination of variables was calculated. The results demonstrated that MH microbubble size and antimicrobial exposure time were the key factors that improved growth inhibition. Additionally, the use of a high-density stainless steel mesh nozzle to produce MH microbubbles significantly enhanced inhibition of bacterial growth, with smaller microbubble sizes showing better inhibition (p < 0.05). Complete bacterial elimination (3.5 log) was achieved using either waterproof or foam dressings with three of nozzle layers (producing the microbubbles of 0.0016 mm) and 3 mL of microbubbles over 24 h.

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麦卢卡蜂蜜微泡颗粒结构优化对铜绿假单胞菌生长的抑制作用。

本研究旨在优化麦卢卡蜂蜜（MH）微泡对铜绿假单胞菌（P. aeruginosa）感染的抗菌效果。实验设计包括三种高密度不锈钢网层、两种敷料、两种用量和三种抗菌药物暴露时间产生的三种不同MH微泡粒径。计算各变量组合下铜绿假单胞菌的细菌存活率。结果表明，MH微泡大小和抗菌暴露时间是提高生长抑制作用的关键因素。此外，使用高密度不锈钢网状喷嘴产生MH微泡显著增强了对细菌生长的抑制作用，微泡尺寸越小，抑制作用越好(p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.