Comparative Transcriptome Analysis Reveals Epithelial Growth Factor Receptor (EGFR) Pathway and Secreted C-Type Lectins as Essential Drivers of Leg Regeneration in Periplaneta americana.

IF 2.9 2区农林科学 Q1 ENTOMOLOGY

Insects Pub Date : 2025-09-05 DOI:10.3390/insects16090934

Xiaoxuan Liu, Nan Sun, Xiaojuan Wu, Jiajia Wu, Shuqi Xian, Dayong Wang, Yechun Pei

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引用次数: 0

Abstract

The American cockroach (Periplaneta americana) serves as an exemplary model for regeneration research due to its exceptional regenerative capabilities, particularly in appendage regeneration. In this study, regenerated coxa tissue underwent histological analysis through H & E straining. Microscopic examination revealed the progression of regeneration. To elucidate the underlying mechanisms, a comparative transcriptomic analysis was conducted between regenerating legs and non-amputated control legs. This analysis identified 2343 differentially expressed genes (DEGs) between 0 days post-amputation (0 dpa) and 7 dpa, 2963 DEGs between 14 dpa and 0 dpa, and 3135 DEGs between 14 dpa and 7 dpa. Significantly, several DEGs are associated with growth- or regeneration-related processes, including extracellular matrix (different collagen, Pro-resilin isoforms, integrin beta (itgb) and matrix metalloproteinase (mmp)), immune-related genes (Toll-like receptor 13 (tlr13), defensin (def), drosomycin-like defensin (dld), Polyphenoloxidases2 (ppo2), cytochrome P450 (p450), peptidoglycan recognition protein (pgrp) and secreted C-type lectin (sClec)), insulin-like growth factor (IGF) and Epidermal Growth Factor Receptor (EGFR). Functional validation through RNA interference (RNAi) further suggested that EGFR and a specific C-type lectin (Regenectin) regulate leg regeneration in Periplaneta americana. These findings enhance our understanding of the molecular mechanisms governing regeneration in this species.

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比较转录组分析揭示上皮生长因子受体（EGFR）途径和分泌的c型凝集素是美洲大蠊腿部再生的重要驱动因素。

美洲大蠊（美洲大蠊）由于其特殊的再生能力，特别是在附属物再生方面，可以作为再生研究的典范。在本研究中，对再生的髋部组织进行了h&e拉伸的组织学分析。显微镜检查显示再生的进展。为了阐明潜在的机制，在再生腿和未截肢的对照腿之间进行了比较转录组学分析。在截肢后0天（0 dpa）至7 dpa之间鉴定出2343个差异表达基因，在14 dpa至0 dpa之间鉴定出2963个差异表达基因，在14 dpa至7 dpa之间鉴定出3135个差异表达基因。值得注意的是，一些deg与生长或再生相关的过程有关，包括细胞外基质(不同的胶原蛋白、前弹性蛋白异构体、整合素β （itgb)和基质金属蛋白酶（mmp））、免疫相关基因（toll样受体13 （tlr13）、防御素（def）、促生霉素样防御素（dld）、多酚氧化酶2 （ppo2）、细胞色素P450 （P450）、肽聚糖识别蛋白（pgrp）和分泌的c型凝集素（sClec））、胰岛素样生长因子（IGF）和表皮生长因子受体（EGFR）。通过RNA干扰（RNAi）的功能验证进一步表明，EGFR和特定的c型凝集素（Regenectin）调节美洲大蠊的腿再生。这些发现增强了我们对该物种再生的分子机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insects Agricultural and Biological Sciences-Insect Science

CiteScore

5.10

自引率

10.00%

发文量

1013

审稿时长

21.77 days

期刊介绍： Insects (ISSN 2075-4450) is an international, peer-reviewed open access journal of entomology published by MDPI online quarterly. It publishes reviews, research papers and communications related to the biology, physiology and the behavior of insects and arthropods. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.