The Dose-Dependent Influence of Type 2 Resistant Starch on Gut Microbial Communities and Metabolic Outputs: An In Vitro Simulation.

IF 5.1 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Foods Pub Date : 2025-09-19 DOI:10.3390/foods14183255

Huowang Zheng, Fangshu Shi, Jinjun Li, Xiangyu Bian, Shuisheng Wu, Xiaoqiong Li

{"title":"The Dose-Dependent Influence of Type 2 Resistant Starch on Gut Microbial Communities and Metabolic Outputs: An In Vitro Simulation.","authors":"Huowang Zheng, Fangshu Shi, Jinjun Li, Xiangyu Bian, Shuisheng Wu, Xiaoqiong Li","doi":"10.3390/foods14183255","DOIUrl":null,"url":null,"abstract":"This study systematically investigated the dose-response relationship of resistant starch type 2 (RS2; Hi-maize 260; 0-15 g/L) on gut microbial composition, short-chain fatty acid (SCFA)/gas output, and tryptophan catabolism using an in vitro fermentation model. The highest RS2 concentration (15 g/L) elicited optimal metabolic outcomes, including maximal SCFA production; significant H2S reduction; and redirected tryptophan metabolism from potentially detrimental indoles toward neuroprotective metabolites. Microbial profiling revealed dose-dependent enrichment of saccharolytic taxa (Bifidobacterium, Lactobacillus) with concomitant suppression of proteolytic pathobionts (e.g., Escherichia-Shigella). Correlation analyses revealed strong positive associations between beneficial microbes and both SCFAs and neuroprotective metabolites, whereas pathogenic taxa correlated inversely with these compounds. Collectively, these findings establish that functionally relevant microbiome modulation requires a sufficiently high, dose-tailored intake of RS2, providing a rational basis for precision dietary strategies aimed at improving host metabolic and gut health.","PeriodicalId":12386,"journal":{"name":"Foods","volume":"14 18","pages":""},"PeriodicalIF":5.1000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12469863/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foods","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/foods14183255","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study systematically investigated the dose-response relationship of resistant starch type 2 (RS2; Hi-maize 260; 0-15 g/L) on gut microbial composition, short-chain fatty acid (SCFA)/gas output, and tryptophan catabolism using an in vitro fermentation model. The highest RS2 concentration (15 g/L) elicited optimal metabolic outcomes, including maximal SCFA production; significant H₂S reduction; and redirected tryptophan metabolism from potentially detrimental indoles toward neuroprotective metabolites. Microbial profiling revealed dose-dependent enrichment of saccharolytic taxa (Bifidobacterium, Lactobacillus) with concomitant suppression of proteolytic pathobionts (e.g., Escherichia-Shigella). Correlation analyses revealed strong positive associations between beneficial microbes and both SCFAs and neuroprotective metabolites, whereas pathogenic taxa correlated inversely with these compounds. Collectively, these findings establish that functionally relevant microbiome modulation requires a sufficiently high, dose-tailored intake of RS2, providing a rational basis for precision dietary strategies aimed at improving host metabolic and gut health.

Abstract Image

查看原文本刊更多论文

2型抗性淀粉对肠道微生物群落和代谢输出的剂量依赖性影响：体外模拟

本研究采用体外发酵模型，系统研究了抗性淀粉2型（RS2; high -maize 260; 0-15 g/L）对肠道微生物组成、短链脂肪酸(SCFA)/气体输出和色氨酸分解代谢的量效关系。最高浓度的RS2 （15 g/L）诱导了最佳的代谢结果，包括最大的SCFA产量；H2S显著减少；并将色氨酸代谢从潜在有害的吲哚转向神经保护代谢物。微生物分析显示，糖分解分类群（双歧杆菌、乳酸杆菌）的富集与蛋白分解病原菌（如埃希氏志贺氏菌）的抑制呈剂量依赖性。相关分析显示，有益微生物与SCFAs和神经保护代谢物之间存在强烈的正相关，而致病分类群与这些化合物呈负相关。总之，这些研究结果表明，功能相关的微生物组调节需要足够高的、量身定制的RS2摄入量，为旨在改善宿主代谢和肠道健康的精确饮食策略提供了合理的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Foods Immunology and Microbiology-Microbiology

CiteScore

7.40

自引率

15.40%

发文量

3516

审稿时长

15.83 days

期刊介绍： Foods (ISSN 2304-8158) is an international, peer-reviewed scientific open access journal which provides an advanced forum for studies related to all aspects of food research. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists, researchers, and other food professionals to publish their experimental and theoretical results in as much detail as possible or share their knowledge with as much readers unlimitedly as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: manuscripts regarding research proposals and research ideas will be particularly welcomed electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material we also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds