Dual Blockade of PI3K and EGFR Pathways by Flavonoids from Idesia polycarpa Maxim Cake Meal: Valorization of Agro-Industrial Waste for NSCLC Therapy.

IF 5.1 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Foods Pub Date : 2025-09-22 DOI:10.3390/foods14183278

Zhenyu Yang, Kai Luo, Dan Chen, Lei Dou, Xiufang Huang, Jianquan Kan

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引用次数: 0

Abstract

Efficient utilization of food industry waste supports sustainable development. Idesia polycarpa Maxim cake meal (an oil-processing by-product) is rich in bioactive flavonoids, but the refined purification, anti-non-small cell lung cancer (NSCLC) activity, and mechanism of its total flavonoids (IPTF) remain unclear-restricting high-value use. This study optimized IPTF purification via polyamide resin gradient elution and characterized its chemical composition by HPLC/LC-MS. In vitro assays assessed IPTF's effects on A549 cell proliferation, migration, invasion, colony formation, and apoptosis; network pharmacology and molecular docking predicted mechanisms, validated via Western blotting for key signaling pathways. Results showed IPTF purity was significantly improved after purification; HPLC/LC-MS identified rutin, quercetin, and six minor components as key constituents. IPTF inhibited A549 proliferation, and network pharmacology indicated it synergistically targets the PI3K/AKT and EGFR-MAPK pathways-validated by reduced phosphorylation of p-AKT, p-EGFR, and p-ERK. This work offers a novel strategy for I. polycarpa cake meal valorization and highlights IPTF's potential as a multi-target natural candidate for NSCLC therapy.

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黄酮类化合物对PI3K和EGFR通路的双重阻断：农用工业废物对非小细胞肺癌治疗的价值

有效利用食品工业废弃物支持可持续发展。油品加工副产物芦笋饼粕中含有丰富的生物活性黄酮类化合物，但其精制纯化、抗非小细胞肺癌（NSCLC）活性及其作用机制尚不清楚，制约了芦笋饼粕的高价值利用。本研究优化了聚酰胺树脂梯度洗脱纯化IPTF的工艺，并采用HPLC/LC-MS对其化学成分进行了表征。体外实验评估IPTF对A549细胞增殖、迁移、侵袭、集落形成和凋亡的影响；网络药理学和分子对接预测机制，通过Western blotting验证关键信号通路。结果表明，纯化后IPTF纯度显著提高；HPLC/LC-MS鉴定出芦丁、槲皮素和6个次要成分为主要成分。IPTF抑制A549的增殖，网络药理学表明它协同作用于PI3K/AKT和EGFR-MAPK通路，通过降低p-AKT、p-EGFR和p-ERK的磷酸化来验证。这项工作提供了一种新的策略，为聚乳酸蛋白饼粉增值，并突出IPTF作为NSCLC治疗的多靶点天然候选物的潜力。

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来源期刊

Foods Immunology and Microbiology-Microbiology

CiteScore

7.40

自引率

15.40%

发文量

3516

审稿时长

15.83 days

期刊介绍： Foods (ISSN 2304-8158) is an international, peer-reviewed scientific open access journal which provides an advanced forum for studies related to all aspects of food research. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists, researchers, and other food professionals to publish their experimental and theoretical results in as much detail as possible or share their knowledge with as much readers unlimitedly as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: manuscripts regarding research proposals and research ideas will be particularly welcomed electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material we also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds