Safflower Polysaccharides Alleviate TNBS-Induced Colitis by Modulating Gut Immunity.

IF 5.1 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Foods Pub Date : 2025-09-14 DOI:10.3390/foods14183199

Chao Jiang, Furong Zhu, Shabaaiti Aimaier, Liang Zhang, Md Hasan Ali, Furong Fan, Yating Lu, Mengwei Jia, Dongsen Wu, Haipeng Yin, Jianwang Wei, Shenghui Chu, Min Liu

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引用次数: 0

Abstract

This study aims to investigate the potential immunological mechanisms by which Safflower polysaccharides (SPSs) regulate colitis. The therapeutic effect of SPSs on colitis was investigated by trinitrobenzene sulfonic acid (TNBS)-induced rats model, TNF-α-stimulated Caco-2 cells, LPS-induced THP-1 cell model, and a co-culture model of Caco-2 and THP-1. The results demonstrated that SPSs effectively ameliorated clinical symptoms, reduced the expression of pro-inflammatory factors, restored colonic pathological damage, regulated the body's immunity, and inhibited intestinal macrophage M1 polarization in vivo. In vitro, SPSs could alleviate the inflammatory response of epithelial cells, inhibit macrophage M1 polarization and regulate epithelial-immune cells interaction. Through the experimental study of siRNA-CHI3L1 and r-CHI3L1, it was found that CHI3L1 mediated the interaction between epithelial cells and immune cells. This study demonstrated that SPS can significantly improve clinical symptoms and alleviated colonic damage in TNBS-induced colitis rat models. The underlying mechanisms are associated with STAT3/NF-κB signaling and immunomodulation, where the immunoregulatory effect is based on CHI3L1-mediated epithelial-immune cell interaction mechanisms.

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红花多糖通过调节肠道免疫减轻tnbs诱导的结肠炎。

本研究旨在探讨红花多糖（SPSs）调节结肠炎的潜在免疫机制。采用三硝基苯磺酸（TNBS）诱导大鼠结肠炎模型、TNF-α-刺激Caco-2细胞模型、lps诱导THP-1细胞模型、Caco-2与THP-1共培养模型研究SPSs对结肠炎的治疗作用。结果表明，SPSs在体内可有效改善临床症状，降低促炎因子表达，恢复结肠病理损伤，调节机体免疫，抑制肠道巨噬细胞M1极化。在体外，SPSs可以减轻上皮细胞的炎症反应，抑制巨噬细胞M1极化，调节上皮-免疫细胞相互作用。通过siRNA-CHI3L1和r-CHI3L1的实验研究发现，CHI3L1介导了上皮细胞与免疫细胞的相互作用。本研究表明，SPS能显著改善tnbs诱导的大鼠结肠炎模型的临床症状，减轻结肠损伤。潜在的机制与STAT3/NF-κB信号传导和免疫调节有关，其中免疫调节作用是基于chi3l1介导的上皮-免疫细胞相互作用机制。

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来源期刊

Foods Immunology and Microbiology-Microbiology

CiteScore

7.40

自引率

15.40%

发文量

3516

审稿时长

15.83 days

期刊介绍： Foods (ISSN 2304-8158) is an international, peer-reviewed scientific open access journal which provides an advanced forum for studies related to all aspects of food research. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists, researchers, and other food professionals to publish their experimental and theoretical results in as much detail as possible or share their knowledge with as much readers unlimitedly as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: manuscripts regarding research proposals and research ideas will be particularly welcomed electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material we also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds