Exploring the effect of capsaicin on gene expression and chemotherapy sensitization in gastric cancer cells.

IF 3.4 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

European Journal of Medical Research Pub Date : 2025-09-26 DOI:10.1186/s40001-025-03127-9

Weijian Meng, Kun Xie, Zhihong Qi, Rui Zhang, Jie Shen

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引用次数: 0

Abstract

Background: Capsaicin, phytochemical component in red hot chili peppers, has previously been demonstrated to exhibit antitumor effect in various cancer type. However, the deep biological function and molecular mechanism of capsaicin was still uncertain.

Methods: This study applied cell viability assay, apoptosis assay, Transwell assay, immunofluorescence, qPCR, western blot assay and high-throughput RNA sequencing to explore the effect of capsaicin on gene expression and chemotherapy sensitization in gastric cancer cells.

Results: Capsaicin could significantly inhibit cell viability and induce apoptosis in both AGS and HGC-27 gastric cancer cell line. Through high-throughput RNA sequencing, capsaicin exhibited inhibiting role in DNA repair, DNA replication and chromosome assemble pathway. qPCR assay and western blot validated that capsaicin could inhibit expression of the key enzymes (FEN1, LIG1 and PARP1) in DNA damage response and chemotherapy resistance. In vitro assay demonstrated that capsaicin could significantly induce cyto-toxicity and chemotherapy-induced DNA damage of 5-FU and Oxalipatin at low dose. Immunofluorescence analysis revealed that capsaicin inhibited both the expression and nuclear accumulation of PARP1 in AGS cells treated with 5-FU or oxaliplatin.

Conclusions: Capsaicin could inhibit DNA repair, thereby inhibited cell viability and improved the sensitivity of chemotherapy in gastric cancer cells.

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探讨辣椒素对胃癌细胞基因表达及化疗致敏的影响。

背景：辣椒素是红辣椒中的一种植物化学成分，已被证实对多种癌症具有抗肿瘤作用。然而，辣椒素的深层生物学功能和分子机制尚不清楚。方法：采用细胞活力法、凋亡法、Transwell法、免疫荧光法、qPCR法、western blot法、高通量RNA测序等方法，探讨辣椒素对胃癌细胞基因表达及化疗致敏的影响。结果：辣椒素对胃癌AGS和HGC-27细胞均有明显抑制细胞活力和诱导凋亡的作用。通过高通量RNA测序，辣椒素在DNA修复、DNA复制和染色体组装途径中显示出抑制作用。qPCR和western blot验证了辣椒素可以抑制DNA损伤应答和化疗耐药过程中关键酶FEN1、LIG1和PARP1的表达。体外实验表明，辣椒素在低剂量下可显著诱导5-FU和奥沙利铂的细胞毒性和化疗诱导的DNA损伤。免疫荧光分析显示，辣椒素抑制5-FU或奥沙利铂处理的AGS细胞中PARP1的表达和核积累。结论：辣椒素可抑制胃癌细胞DNA修复，从而抑制细胞活力，提高化疗敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Medical Research 医学-医学：研究与实验

CiteScore

3.20

自引率

0.00%

发文量

247

审稿时长

>12 weeks

期刊介绍： European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.