Detection of Treponema pallidum tpp47 DNA in clinical samples of syphilis patients.

IF 3.4 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

European Journal of Medical Research Pub Date : 2025-09-26 DOI:10.1186/s40001-025-03148-4

Yongjun Meng, Ling Yang, Yu Fu, Si Li, Krishna Hamal, Donghua Liu

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引用次数: 0

Abstract

Backgrounds: To investigate the relationship between the presence of Treponema pallidum DNA in saliva, serum, and cerebrospinal fluid (CSF) in patients with different stages of syphilis.

Patients and methods: From 2020 to 2024, clinical samples, including saliva, serum, and CSF, were collected from 740 patients diagnosed with syphilis at the First Affiliated Hospital of Guangxi Medical University. Primer sequences targeting the Treponema pallidum gene tpp47 were designed for nested PCR (nPCR).

Results: A total of 1023 samples were collected from 740 patients with syphilis, including 20 primary syphilis, 96 secondary syphilis, 90 neurosyphilis, and 534 latent syphilis. The overall detection rates of Treponema pallidum DNA in saliva, serum, and CSF were 13.6% (36/264), 3.1% (17/543), and 5.5% (12/216), and in secondary syphilis 40.0% (26/65), 3.4% (2/59), and 7.1% (1/14), all of which were significantly different (P < 0.001). The detection rates in secondary syphilis were 40.0% (26/65), 3.4% (2/59), and 7.1% (1/14), which were significantly different (P < 0.001) from those observed in the other categories. Notably, no significant discrepancies were observed between the three clinical samples from patients with primary, neurosyphilis, and latent syphilis (P > 0.05).

Conclusion: The Treponema pallidum DNA is most abundant in the saliva of syphilis patients, indicating a potential risk of saliva-mediated transmission of the disease.

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梅毒患者临床标本中梅毒螺旋体tpp47 DNA的检测。

背景：探讨梅毒螺旋体DNA在不同阶段梅毒患者唾液、血清和脑脊液中存在的关系。患者与方法：2020 - 2024年，采集广西医科大学第一附属医院740例梅毒患者的唾液、血清、脑脊液等临床样本。设计了针对梅毒螺旋体基因tpp47的引物序列，用于巢式PCR （nPCR）。结果：740例梅毒患者共采集标本1023份，其中原发性梅毒20例，继发性梅毒96例，神经梅毒90例，潜伏性梅毒534例。唾液、血清、脑脊液梅毒螺旋体DNA总检出率分别为13.6%（36/264）、3.1%（17/543）、5.5%(12/216)，二期梅毒梅毒螺旋体DNA总检出率分别为40.0%（26/65）、3.4%（2/59）、7.1%(1/14)，差异均有统计学意义（P < 0.05）。结论：梅毒患者唾液中梅毒螺旋体DNA含量最高，提示梅毒存在唾液传播的潜在风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Medical Research 医学-医学：研究与实验

CiteScore

3.20

自引率

0.00%

发文量

247

审稿时长

>12 weeks

期刊介绍： European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.