In-depth analysis of metabolic hormones and inflammatory markers following Roux-en-Y gastric bypass in humans and rodents: similarities and differences

IF 7.4 3区医学 Q1 ENDOCRINOLOGY & METABOLISM

Diabetes research and clinical practice Pub Date : 2025-09-24 DOI:10.1016/j.diabres.2025.112923

Simon Kloock , Lukas Scheller , Julia Hasinger , Ilja Balonov , Max Kurlbaum , Martin Fassnacht , Ann-Cathrin Koschker , Florian Seyfried , Ulrich Dischinger

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引用次数: 0

Abstract

Background

Bariatric surgery causes changes in the levels of metabolically active hormones that control energy expenditure. This study aims to (1) validate a Roux-en-Y gastric bypass (RYGB) rat model in comparison to RYGB operated humans and (2) investigate the correlation of amino acids with GLP-1 and PYY levels in both species.

Methods

Fasting plasma samples were derived from the randomized controlled WAS trial (NCT01352403; RYGB = 20, Controls = 17) at baseline and after 12 months and from male Wistar rats with diet-induced obesity seven weeks after surgery (RYGB = 12, sham surgery = 12). 18 peptide hormones and 21 amino acids were measured using magnetic multiplex assays, ELISA and LC-MS/MS.

Results

Levels of GLP-1 and PYY3-36 were found to be significantly lower in humans after RYGB (both p < 0.001), while in rats a trend towards an increase was observed. Fasting insulin was found to be lower in humans (p < 0.001) and rats (p < 0.01) after RYGB. Leptin was significantly lower in humans (p < 0.001) and rats (p < 0.05) after RYGB. The cytokines IL-6 and MCP-1 were significantly lower in humans (p < 0.01, p < 0.05), but unchanged in rats after RYGB. Interestingly, GLP-1 levels in humans before RYGB correlated positively with the weight change after 12 months (Pearson’s r = 0.733; p < 0.05). Leucine showed a positive correlation with GLP-1 levels 12 months after RYGB in humans (Pearson’s r = 0.588; p < 0.05), but not in rats.

Conclusion

Preoperative GLP-1 levels in humans correlate with weight loss after RYGB and could potentially be predictive. The investigated rat model shows largely comparable patterns of incretins and adipokines. Given its physiological similarity, this model is suitable for testing pharmacological agents that mimic anorexigenic hormones, potentially guiding novel treatments for severe obesity.

查看原文本刊更多论文

人类和啮齿类动物Roux-en-Y胃旁路术后代谢激素和炎症标志物的深入分析：异同。

背景：减肥手术引起控制能量消耗的代谢活性激素水平的变化。本研究旨在验证Roux-en-Y胃旁路（RYGB）大鼠模型与RYGB操作的人模型的有效性，并研究两种动物中氨基酸与GLP-1和PYY水平的相关性。方法：空腹血浆样本来自随机对照WAS试验（NCT01352403; RYGB = 20，对照 = 17）基线和12 个月后，以及术后7周饮食性肥胖的雄性Wistar大鼠（RYGB = 12，假手术 = 12）。采用磁复吸法、ELISA和LC-MS/MS检测18种肽类激素和21种氨基酸。结果：在RYGB后，GLP-1和PYY3-36的水平被发现显著降低（p均为 ）。结论：术前GLP-1水平与RYGB后体重减轻有关，可能具有预测作用。所研究的大鼠模型显示肠促胰岛素和脂肪因子在很大程度上具有可比性。鉴于其生理上的相似性，该模型适用于测试模拟厌氧性激素的药理学药物，可能指导严重肥胖的新治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diabetes research and clinical practice 医学-内分泌学与代谢

CiteScore

10.30

自引率

3.90%

发文量

862

审稿时长

32 days

期刊介绍： Diabetes Research and Clinical Practice is an international journal for health-care providers and clinically oriented researchers that publishes high-quality original research articles and expert reviews in diabetes and related areas. The role of the journal is to provide a venue for dissemination of knowledge and discussion of topics related to diabetes clinical research and patient care. Topics of focus include translational science, genetics, immunology, nutrition, psychosocial research, epidemiology, prevention, socio-economic research, complications, new treatments, technologies and therapy.