Size-selective particle separation combined with fluorescence excitation module for real-time detection of airborne microorganisms: a feasibility study

Abstract

Over the past few decades, the detection of airborne pathogens in various indoor and outdoor settings has emerged as a crucial area of research and development. Bioaerosols, stemming from natural or industrial sources and comprising airborne organisms or their fragments, pose potential public and industrial health risks. Hence, there is a growing emphasis on achieving early and dependable detection methods for these pathogens across different environments. This project investigates some possibilities for developing cost-effective “first alert” technology capable of detecting airborne bacteria, fungi and pollen in real time. The proposed approach shows significant promise as an initial alert system capable of alerting users to the possible presence of pathogens or allergens in the air, allowing for the timely implementation of personal protective measures. Although the device cannot differentiate between specific types of bacteria, fungi or pollen, it effectively collects and retains them in a liquid sample. This allows for their precise characterization to be conducted in the nearest laboratory. Subsequently, decisions regarding the retention or removal of protective equipment can be made based on the laboratory results, with further guidance sought from public health specialists as needed.

Graphical abstract