{"title":"Construction and Performance of Nano Dual-Signal Composite Detection System for Foodborne Pathogen Detection in Meat","authors":"Yuhan Gao, Hang Ji","doi":"10.1007/s12161-025-02780-4","DOIUrl":null,"url":null,"abstract":"<div><p>Food safety concerns everyone, and <i>Staphylococcus aureus</i> is a common foodborne pathogen with a complex and slow detection process. To improve the detection speed and accuracy of pathogenic bacteria in fresh meat products, a dual-signal composite rapid detection system consisting of upconversion fluorescence detection and magnetic nano detection is developed. When the ratio of upconversion nanomaterials to magnetic conversion nanomaterials was 10:9, the processing time of the detection system was 20 min. When the stabilizer was added to the detection system, the detection accuracy reached more than 90%. The designed method took the shortest detection time, taking only 1 day to complete, while the traditional plate counting method took 5 days and the nano rapid detection method took 2 days. When the detection system designed for the study detects <i>Staphylococcus aureus</i>, the detection accuracy was similar to that of the traditional plate counting, with a relative standard deviation of less than 10% and could be reduced to around 3%. In this paper, a rapid detection system for dual-signal composite is developed based on upconversion fluorescence and magnetic nanomaterials. When the concentration was 1 × 10<sup>3</sup>, the detection concentration, recovery rate, and detection time of the designed method were 0.95 ± 0.02 × 10<sup>3</sup> CFU/mL, 95%, and 1 day, respectively. However, under the same conditions, the nano rapid detection method had a detection concentration, recovery rate, and detection time of 0.91 ± 0.04 × 10<sup>3</sup> CFU/mL, 91%, and 2 days, respectively. The designed method is superior to existing methods on detection concentration, recovery rate, and detection time. The <i>Staphylococcus aureus</i> detection system effectively accelerates the detection speed of fresh meat products while ensuring detection accuracy.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"18 7","pages":"1228 - 1242"},"PeriodicalIF":3.0000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-025-02780-4","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Food safety concerns everyone, and Staphylococcus aureus is a common foodborne pathogen with a complex and slow detection process. To improve the detection speed and accuracy of pathogenic bacteria in fresh meat products, a dual-signal composite rapid detection system consisting of upconversion fluorescence detection and magnetic nano detection is developed. When the ratio of upconversion nanomaterials to magnetic conversion nanomaterials was 10:9, the processing time of the detection system was 20 min. When the stabilizer was added to the detection system, the detection accuracy reached more than 90%. The designed method took the shortest detection time, taking only 1 day to complete, while the traditional plate counting method took 5 days and the nano rapid detection method took 2 days. When the detection system designed for the study detects Staphylococcus aureus, the detection accuracy was similar to that of the traditional plate counting, with a relative standard deviation of less than 10% and could be reduced to around 3%. In this paper, a rapid detection system for dual-signal composite is developed based on upconversion fluorescence and magnetic nanomaterials. When the concentration was 1 × 103, the detection concentration, recovery rate, and detection time of the designed method were 0.95 ± 0.02 × 103 CFU/mL, 95%, and 1 day, respectively. However, under the same conditions, the nano rapid detection method had a detection concentration, recovery rate, and detection time of 0.91 ± 0.04 × 103 CFU/mL, 91%, and 2 days, respectively. The designed method is superior to existing methods on detection concentration, recovery rate, and detection time. The Staphylococcus aureus detection system effectively accelerates the detection speed of fresh meat products while ensuring detection accuracy.
期刊介绍：
Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.
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