Biodegradation of chloramphenicol by Aspergillus neoflavipes AJC3

IF 2.5 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2025-05-28 DOI:10.1007/s11696-025-04123-3

Raveena Ann Alex, Jayanthi Abraham

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Abstract

The possibility of environmental contamination associated with chloramphenicol requires special attention. The primary challenges in the biodegradation of chloramphenicol from the contaminated environment are obtaining effective antibiotic-degrading microbes of pure cultures and determining the entire metabolic pathway involved in efficient degradation. The potent strain Aspergillus neoflavipes AJC3 was able to degrade 98.70% of chloramphenicol at a concentration of 50 mg/L within 8 d. The biodegradation pattern of chloramphenicol was evaluated by employing high-performance liquid chromatography (HPLC) and validated through gas chromatography mass spectroscopy (GC–MS), liquid chromatography mass spectrometry (LC–MS) and Fourier transform infrared spectroscopy (FTIR). Three metabolic compounds were identified during the chloramphenicol biodegradation process and the degradation pathway involved zero-order and first-order kinetics model according to the kinetics energy. And to evaluate the effects of chloramphenicol in aquatic environment, toxicity studies were conducted using the nauplii and cysts of Artemia salina and the nauplii mortality was evaluated at 24 h.

Graphical abstract

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新黄曲霉AJC3对氯霉素的生物降解

与氯霉素有关的环境污染的可能性需要特别注意。从污染环境中生物降解氯霉素的主要挑战是获得有效的纯培养抗生素降解微生物，并确定参与有效降解的整个代谢途径。新黄曲霉AJC3在8 d内对浓度为50 mg/L的氯霉素的降解率为98.70%。采用高效液相色谱（HPLC）评价菌株对氯霉素的生物降解模式，并通过气相色谱-质谱（GC-MS）、液相色谱-质谱（LC-MS）和傅里叶变换红外光谱（FTIR）进行验证。在氯霉素生物降解过程中鉴定出3种代谢产物，降解途径根据动力学能量分为零级和一级动力学模型。为了评价氯霉素对水生环境的影响，我们对盐渍蒿（Artemia salina）的nauplii和囊肿进行了毒性研究，并在24 h时评估了nauplii的死亡率

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来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.