Doxorubicin and cisplatin dual-loaded mesoporous silica nanoparticles coated with polyacrylic acid brushes to enhance anticancer activities

IF 3.2 4区材料科学 Q2 CHEMISTRY, APPLIED

Journal of Porous Materials Pub Date : 2025-02-27 DOI:10.1007/s10934-025-01781-2

Amal Siraj, Anas Aljarbou, Nasser B. Alsaleh, Amal Alfawaz, Abeer Beagan, Khalid Alotaibi, Abdullah Alswieleh

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Abstract

In this study, mesoporous silica nanoparticles (MSNs) were synthesized via a sol–gel process and functionalized with pH-responsive polyacrylic acid (PAA) brushes through surface-initiated atom transfer radical polymerization. The resulting nanoparticles, with an average diameter of approximately 100 nm as determined by SEM and TEM, exhibited pH-sensitive swelling and shrinking behavior, confirmed by DLS analysis. Fourier Transform Infrared Spectroscopy (FT-IR) verified the successful conjugation of PAA through the appearance of characteristic carboxylate absorption bands. The MSNs were evaluated as nanocarriers for cisplatin (CP@MSNs-PAA) and as dual nanocarriers for cisplatin and doxorubicin (CP/DOX@MSNs-PAA), achieving high drug entrapment efficiencies (~ 98%). Drug release studies demonstrated near-complete release of cisplatin and optimized release of DOX (49.94%) under mildly acidic conditions (pH 6.5). Energy-Dispersive X-ray Spectroscopy (EDX) confirmed the effective loading and release of both drugs, as evidenced by changes in elemental composition before and after release. In vitro cytotoxicity assays indicated the biocompatibility of unloaded MSNs-PAA, while CP/DOX@MSNs-PAA exhibited significantly enhanced cytotoxic effects, highlighting the synergistic therapeutic potential of the dual drug-loaded system.

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多柔比星和顺铂双载介孔二氧化硅纳米颗粒包覆聚丙烯酸刷增强抗癌活性

本研究采用溶胶-凝胶法合成介孔二氧化硅纳米颗粒（MSNs），并通过表面引发原子转移自由基聚合，在ph响应型聚丙烯酸（PAA）刷上进行功能化。经扫描电镜和透射电镜测定，所得纳米颗粒的平均直径约为100 nm，并通过DLS分析证实了其对ph敏感的膨胀和收缩行为。傅里叶变换红外光谱（FT-IR）通过羧酸盐特征吸收带的出现证实了PAA的成功偶联。msn被评价为顺铂的纳米载体（CP@MSNs-PAA）和顺铂和阿霉素的双纳米载体（CP/DOX@MSNs-PAA），具有很高的药物包封效率（~ 98%）。药物释放研究表明，在轻度酸性条件下（pH 6.5），顺铂几乎完全释放，DOX的最佳释放（49.94%）。能量色散x射线光谱（EDX）证实了两种药物的有效加载和释放，释放前后元素组成的变化证明了这一点。体外细胞毒性实验表明，空载MSNs-PAA具有生物相容性，而CP/DOX@MSNs-PAA具有显著增强的细胞毒性作用，突出了双重药物负载系统的协同治疗潜力。

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来源期刊

Journal of Porous Materials 工程技术-材料科学：综合

CiteScore

4.80

自引率

7.70%

发文量

203

审稿时长

2.6 months

期刊介绍： The Journal of Porous Materials is an interdisciplinary and international periodical devoted to all types of porous materials. Its aim is the rapid publication of high quality, peer-reviewed papers focused on the synthesis, processing, characterization and property evaluation of all porous materials. The objective is to establish a unique journal that will serve as a principal means of communication for the growing interdisciplinary field of porous materials. Porous materials include microporous materials with 50 nm pores. Examples of microporous materials are natural and synthetic molecular sieves, cationic and anionic clays, pillared clays, tobermorites, pillared Zr and Ti phosphates, spherosilicates, carbons, porous polymers, xerogels, etc. Mesoporous materials include synthetic molecular sieves, xerogels, aerogels, glasses, glass ceramics, porous polymers, etc.; while macroporous materials include ceramics, glass ceramics, porous polymers, aerogels, cement, etc. The porous materials can be crystalline, semicrystalline or noncrystalline, or combinations thereof. They can also be either organic, inorganic, or their composites. The overall objective of the journal is the establishment of one main forum covering the basic and applied aspects of all porous materials.