Enhancing high-value metabolite production and asparaginase activity in Haematococcus pluvialis: impact of different organic carbon sources and feeding strategies

IF 4.1 4区工程技术 Q3 ENERGY & FUELS

Biomass Conversion and Biorefinery Pub Date : 2025-03-25 DOI:10.1007/s13399-025-06779-z

Laenne Barbara Silva de Moraes, Géssica Cavalcanti Pereira Mota, Alejandro Molina-Miras, Asterio Sánchez-Mirón, María del Carmen Cerón-García, Alfredo Olivera Gálvez, Ranilson de Souza Bezerra, Francisco García-Camacho

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引用次数: 0

Abstract

This study explores modifications in sources of organic carbon and strategies for adding them to enhance the metabolite production in Haematococcus pluvialis. Thus, the combined effects of two factors were investigated: the type of single organic carbon source (sodium acetate, AC; molasses, MS; glycerol, GLY) and organic carbon feeding strategy, either single pulse (SPF) or multi-pulse (MPF). This study achieved enhanced biomass yields of H. pluvialis using SPF-GLY and MPF-AC. The incorporation of glycerol and molasses significantly enhanced the biosynthesis of proteins, fatty acids, and astaxanthin. Notably, SPF-GLY achieved the highest astaxanthin content in the biomass (6.89% d.w.). Furthermore, this study reports, for the first time, the activity of the enzyme asparaginase in H. pluvialis. The highest asparaginase activities were observed in SPF-AC and SPF-GLY (245 ± 16 IU mg⁻¹ d.w.). These findings indicate that selecting the appropriate organic carbon source and feeding strategy can optimize metabolite production in H. pluvialis. Moreover, integrating H. pluvialis biomass into biorefinery models could enhance the overall utilisation of the valuable compounds produced.

Graphical Abstract

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提高雨红球菌高价值代谢物产量和天冬酰胺酶活性：不同有机碳源和摄食策略的影响

本研究探讨了雨红球菌中有机碳来源的变化以及添加有机碳以提高代谢物产量的策略。因此，研究了单有机碳源（醋酸钠，AC；糖蜜，MS；甘油，GLY）类型和单脉冲（SPF）或多脉冲（MPF）有机碳投料策略两种因素的综合影响。本研究利用SPF-GLY和MPF-AC提高了雨杉的生物量产量。甘油和糖蜜的掺入显著促进了蛋白质、脂肪酸和虾青素的生物合成。其中，SPF-GLY的虾青素含量最高（6.89% d.w.）。此外，本研究还首次报道了雨水蛭中天冬酰胺酶的活性。SPF-AC和SPF-GLY的天冬酰胺酶活性最高（245±16 IU mg−1 d.w）。这些结果表明，选择合适的有机碳源和取食策略可以优化雨杉代谢物的产生。此外，将雨杉生物量整合到生物精炼模型中可以提高所生产的有价值化合物的整体利用率。图形抽象

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来源期刊

Biomass Conversion and Biorefinery Energy-Renewable Energy, Sustainability and the Environment

CiteScore

7.00

自引率

15.00%

发文量

1358

期刊介绍： Biomass Conversion and Biorefinery presents articles and information on research, development and applications in thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion, including all necessary steps for the provision and preparation of the biomass as well as all possible downstream processing steps for the environmentally sound and economically viable provision of energy and chemical products.