Sustainable Optimization of Ohmic Heating–Assisted Extraction of Polyphenolics from Goji Berry (Lycium barbarum L.): LC–MS/MS Profiling and Blueness–Greenness Assessment

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-06-26 DOI:10.1007/s12161-025-02831-w

Harun Kantar, Nesrin Ecem Bayram

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引用次数: 0

Abstract

This study aimed to extract bioactive compounds from goji berry (Lycium barbarum L.) fruit using the ohmic heating–assisted extraction (OHAE) method and optimize the extraction conditions. In this context, firstly, the extraction conditions to be applied for the OHAE method were determined using the response surface method (RSM)-Box Behnken experimental design. During the study, temperature, holding time, and electrical field strength were determined as independent variables, while the total phenolic compound (TPC), total flavonoid compound (TFC), and antioxidant activity (DPPH) were selected as dependent variables. As a result of the optimization, the optimum conditions were determined to be 60 °C for the temperature, 60 min for the holding time, and 45 V/cm for the electrical field strength, and the composite desirability value of the model was 0.90. The highest experimental results for TPC, TFC, and DPPH were found to be 23.83 mg GAE/g, 7.33 mg QE/g, and 24.53 mg TE/g, respectively. In addition, the polyphenolic profiles of all extracts obtained under different process conditions were determined by LC–MS/MS, and rutin was the phenolic detected at the highest rate in the extracts. In addition, the blueness and greenness of the analytical method used in this study were evaluated using the BAGI and ComplexGAPI metrics, respectively. The results indicate that OHAE is an effective and sustainable method for extracting bioactive compounds from goji berries.

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欧姆加热辅助提取枸杞中多酚类物质的可持续优化：LC-MS /MS分析及蓝绿度评价

本研究旨在采用欧姆加热辅助提取法（OHAE）从枸杞果实中提取生物活性物质，并对提取条件进行优化。为此，首先采用响应面法(RSM)-Box Behnken试验设计确定OHAE法适用的提取条件。以温度、保温时间和电场强度为自变量，以总酚类化合物（TPC）、总黄酮类化合物（TFC）和抗氧化活性（DPPH）为因变量。通过优化，确定了温度为60℃，保温时间为60 min，电场强度为45 V/cm的最佳条件，模型的综合理想值为0.90。TPC、TFC和DPPH的最高实验结果分别为23.83 mg GAE/g、7.33 mg QE/g和24.53 mg TE/g。此外，采用LC-MS /MS对不同工艺条件下得到的提取物进行了多酚谱分析，结果表明芦丁是提取物中检出率最高的酚类物质。此外，本研究中使用的分析方法的蓝度和绿度分别使用BAGI和ComplexGAPI指标进行评估。结果表明，OHAE法是一种有效、可持续的提取枸杞中活性物质的方法。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.