Bone Remodeling-Inspired Synthesis of Biomimetic Multi-Doped Calcium Phosphate Nanoparticles for Enhanced Osteoinductive Performance

IF 3.6 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR

Journal of Cluster Science Pub Date : 2025-09-25 DOI:10.1007/s10876-025-02907-x

Mohammed Lakrat, Laura Costa Pinho, Catarina Santos, Maria Helena Fernandes, Allal Barroug, Hassan Noukrati

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Abstract

This work describes a biomimetic approach for synthesizing multi-doped calcium phosphate nanoparticles (CaP NPs) that closely mimic the structure and composition of bone mineral. Inspired by bone remodeling, hydroxyapatite (HAp), used as a combined calcium and phosphorus source, was first dissolved in an acidic medium and then re-precipitated in simulated body fluid (SBF), serving as a physiologically relevant source of trace elements. The results showed that multi-doped amorphous calcium phosphate (ACP) formed within the first few minutes and gradually transformed into poorly crystalline carbonated hydroxyapatite over a 21-day maturation period. This extended observation provides valuable insights into the incorporation of trace elements and their role in the maturation of bone-like mineral phases. The resulting nanoparticles exhibited low crystallinity and nanometric dimensions, along with increased surface area and porosity. They also demonstrated excellent biocompatibility with MG63 osteoblasts and effectively promoted osteogenic responses in human mesenchymal stromal cells. Overall, this strategy offers valuable insights for designing biomimetic CaP NPs inspired by living tissue to support future applications in hard tissue regeneration.

Graphical Abstract

Abstract Image

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骨重塑启发合成仿生多掺杂磷酸钙纳米颗粒增强骨诱导性能

这项工作描述了一种合成多掺杂磷酸钙纳米颗粒（CaP NPs）的仿生方法，该方法非常接近模拟骨矿物质的结构和组成。受骨重塑的启发，羟基磷灰石（HAp）作为钙和磷的组合来源，首先溶解在酸性介质中，然后在模拟体液（SBF）中重新沉淀，作为生理相关的微量元素来源。结果表明，多掺杂的无定形磷酸钙（ACP）在最初的几分钟内形成，并在21天的成熟过程中逐渐转变为低结晶的碳化羟基磷灰石。这一扩展的观察为微量元素的掺入及其在骨样矿物相成熟中的作用提供了有价值的见解。所得到的纳米颗粒具有低结晶度和纳米尺寸，以及增加的表面积和孔隙度。它们还表现出与MG63成骨细胞良好的生物相容性，并能有效促进人间充质基质细胞的成骨反应。总的来说，这一策略为设计受活体组织启发的仿生CaP NPs提供了有价值的见解，以支持未来硬组织再生的应用。图形抽象

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来源期刊

Journal of Cluster Science 化学-无机化学与核化学

CiteScore

6.70

自引率

0.00%

发文量

166

审稿时长

3 months

期刊介绍： The journal publishes the following types of papers: (a) original and important research; (b) authoritative comprehensive reviews or short overviews of topics of current interest; (c) brief but urgent communications on new significant research; and (d) commentaries intended to foster the exchange of innovative or provocative ideas, and to encourage dialogue, amongst researchers working in different cluster disciplines.